All of the articles in this proceedings volume have been presented at the International Conference on Life Science and Biotechnology (ICOLIB) on November 15–16, 2021 at Jember University Indonesia. These articles have been peer-reviewed by the members of the Scientific Committee of ICOLIB and approved...

The Extremely Low Frequency (ELF) magnetic field has an ability to penetrate almost all materials and is non-ionizing radiation so that it can trigger cell proliferation. This study aims to examine the effect of exposure to the 200–300 µT ELF magnetic fields on the growth rate of Edamame plants. This...

Prunus jamasakura Siebold ex Koidz. (hill cherry) is included in the family of Rosaceae and used for ornamental plants. The dormant cherry seed has become an obstacle since it takes a prolonged time to germinate. Seed germination can be induced by the application of gibberellic acid (GA3) and coconut...

Lipase is an enzyme that has various use in industrial sector. However, the enzyme production cost is considerably high mainly due to expensive fermentation substrate. Therefore, it is necessary to develop effective media formulation to increase production of lipase enzyme without reducing the amount...

Sex determination of the embryo during the incubation phase is crucial in the poultry industry. This study focused on solving one of the crucial problems during the incubation phase in the laying poultry breeding industry, namely determining the sex of the embryos. The early stages of research will focus...

Banteng (Bos javanicus) is an endangered native species found in Meru Betiri National Park (MBNP), East Java, Indonesia. This research is aimed to identify the habitat characteristics of banteng at Pringtali feeding ground, Bandealit Resort, MBNP, which is one of the most important habitats for this...

Circular RNAs (circRNAs) represent a milieu of non-coding RNAs that play a major role in gene regulation, development, and carcinogenesis. Circan is a database of cancer associated circular RNAs. As the interest in circRNA increases, Circan provides a systematic annotation of circRNA dysregulation in...

The community nearby palm oil plantations have benefited from the indigenous mushroom growing on decomposed oil palm empty fruit bunches (OPEFB). Some mushrooms are edible, but others are known as non-edible due to their toxicity. There is limited scientific information about the biodiversity of indigenous...

Styrax leave is a part of Styrax plant that can be used as medicine because it contains potential antibacterial and antioxidant bioactive components. Fermentation has been known increasing antibacterial and antioxidant activity of herbal plants. Aspergillus niger is one of fermentation agents that can...

Citric acid is an organic acid and is highly used as a commercial product. The production of citric acid in Indonesia has not met its demand, a cheap and easy method of obtaining raw materials to boost its production is required. Submerged fermentation using Aspergillus niger is a prospective method...

Upeneus molucensis is seawater fishes that are consumed by many people so it leaves a lot of waste (including scales and thornss). On the other hand, the utilization of fish waste is still not much. Whereas, waste contains a lot of amino acids, protein, Calcium and Phosphorus. This study analyzed amino...

The point of this exploration was to decide the benefit of intercropping Indigofera zollingeriana (Iz) and Pennisetum purpureum (Pp) under coconut manor dependent on dry matter yield. This experiment was performed using a completely randomized design (CRD) with six treatment combinations of settlement...

Mosquito salivary glands contain protein substances that can facilitate dengue virus transmission. Those proteins have abilities to induce an immune response and specific antibody production. Previous studies showed that 31 kDa and 67 kDa protein fractions from Aedes albopictus salivary glands are immunogenic...

Apyrase is an enzyme an inhibit platelet aggregation process, capable of degrading ADP in the process blood feeding and mostly found in hematophagous arthropods. While vector’s blood feeding, this apyrase salivary protein is responsible for inhibiting platelet aggregation in the human host, by hydrolyzing...

The population of Javan Langur is continuous decline as the habitat decreases. One of the remaining protected areas of the Javan Langur is Kucur Resort Alas Purwo National Park (APNP). However, the condition of its habitat is still unclear. This study was conducted to predict the distribution and habitat...

The primary mosquito vector of dengue virus is Ae. aegypti, and dengue virus transmitted by saliva mosquito’s during blood feeding. The saliva composed of numerous proteins with capacity to disrupt hemostasis or modulate the host immune response. The previous study identified two immunogenic proteins...

The prospect of using botanical insecticides to control the insect pests is very promising. However, there are some factors limiting their efficacy. Short release rate at the point contact, the inherent volatility and vulnerability to oxidation and ultra-violet light are causing phytochemical changes...

Lymphoma, carcinoma, and benign lesions are common diseases that have to go through several stages to be detected due to their structural similarity. A common way to distinguish these diseases is by analysing them manually based on the cell images at a certain magnification. However, this method still...

In this study, we propose an automatic classification of three common types of lymphoma: (1) lymphoma, (2) benign lesion, and (3) carcinoma using lymphoma cell images magnified by 100x and by 400x. A comparative study was performed to find the best architecture to classify lymphoma cell images using...

Early lymphoma diagnosis is essential to improve the patients’ survival rate and avoid irreversible damage. Immunohistochemistry-based lymphoma diagnostics is an expensive and time-consuming process, especially in developing countries with limited resources. Image-based lymphoma diagnostics might serve...

Toba Banana (Musa acuminata Colla) is a local banana in Toba region, North Sumatera, Indonesia. People only utilize its pulp fruit, then its peels can cause environmental problems. Carbohydrate in banana peel can be utilized as substrate to produce citric acid through fermentation. Citric acid is an...

It has been widely known that sugar palm fruit (Arenga pinnata Merr) contains galactomannan that has antioxidant activity. Antioxidants are compounds that can inhibit free radical formation during oxidation reactions in the body that can cause disease. Antioxidant compounds are commonly found in natural...

Dengue Hemorrhagic Fever (DHF) is a disease generated by dengue virus infection. The dengue virus is transmitted to the host by mosquito vectors. The main and secondary vectors are Aedes aegypti and Aedes albopictus. Dengue virus transmission is mediated by salivary gland proteins that facilitate the...

Many studies have reported that the addition of bromelain enzyme and pineapple extract can increase the growth performance of broiler chickens. However, when the bromelain enzyme was applied to meat, it can digest proteins that cause cell damage. In this study, the addition of the bromelain enzyme with...

Amylase is an enzyme that is used in various industries such as food, paper, and textile industries. Amylase can be produced by microorganisms through fermentation. Rhizopus oryzae is one of microorganisms that can produce amylase through fermentation. Tapioca solid waste has a good potential as a carbon...

Breast cancer is the most common cancer in women. Based on WHO data in 2011, it is estimated that more than 508,000 women worldwide died of breast cancer. In Indonesia, the prevalence of breast cancer is 26.5% of all cancer cases, in which become the second frequent cancer. To date, the treatments for...

Individuals with mandibular prognathism (MP) are characterized by a concave facial profile, and in severe cases, an extremely long face. These clinical characteristics mostly result from the extreme forward growth of the mandible, and are more prevalent in Asians than in Caucasians. MP is a multifactorial...

Hemoglobin contains of heme, which binds iron and protoporphyrin, and globin, which consist of combination of α, β, δ, and γ chain. α-globin globin gene is located in 16p13.3 cytogenetically, and β-globin gene is in 11p15.4. Thalassemia is a blood disorder characterized in the lack or absence of one...

Indonesia is a country that has 40–50 types of essential oil-producing plants out of a total of 80 types of essential oils that enter the world market. There are several essential oil-producing plants that until now have not been used optimally. However, the extraction method of citronella oil in Indonesia...

Diabetes mellitus. is ai global metabolici disease with high morbidity and imortality rates. Many diabetes patients have difficulties lowering their blood glucose levels although they are taking anti-diabetes drugs. Flower of Asian pigeon wings (Clitoria ternatea L) contains flavonoid, anthocyanin, compounds...

Sucrose which is the main product of photosynthesis in plants is resulted from carbon assimilation. The way to produce more sucroses as the sink product has been done a lot recently. However, the way such as overexpression of SPS or harvest environmental engineering needed a lot of samples and a certain...

Patau syndrome, also known as trisomy 13 syndrome, is a syndrome caused by a chromosomal abnormality. This rare syndrome is a lethal disease, where the life expectancy more than 1 year after birth is only less than 15%. The cause of Patau Syndrome is an abnormality on chromosome 13, where the possibilities...

Indonesia is one of the mega-biodiversity countries, with many plant species that are useful as medicine. Red Fruit (Pandanus conoideus Lam), found in Jayawijaya, Papua, is empirically believed to have numerous health benefits due to having a huge amount of bioactive compounds, especially carotenoids....

Screen-printed carbon electrode (SPCE) is one type of electrode commonly used for electrochemical biosensor application. It is fabricated using a screen-printing method, a method in which conductive ink is piled up layer-by-layer using some screen frames. SPCE combines three main electrodes used in the...

The salivary gland proteins of the Anopheles (An.) female mosquito play vital roles in the transmission of Plasmodium into the human host. A comprehensive understanding of Anopheles identity and its related salivary proteins is a key for vector-based malaria control. This research aims to analyse the...

Lactobacillus plantarum is a lactic acid bacterium that can produce lactic acid as well as degrade proteins into soluble proteins, peptides, and amino acids. L. plantarum can also produce bacteriocin, which has antibacterial properties. The goal of this study was to determine the protein levels in fermented...

Mirabilis jalapa is a medicinal plant, which is widely distributed in some tropical countries including in Indonesia. Indicaxanthin, Miraxanthin and Boeravinone F active compounds, which are the family member of Betaxanthins in the M. jalapa plant, are able to inhibit hepcidin and to activate matriptase-2...

The induction of artificial polyploidy has been considered a valuable tool that can improve many plants’ genetics and change their morphological, anatomical, and physiological characteristics. Artemisia cina plants’ polyploidy is induced through shoot culture to increase artemisinin content. This research...

One species of worm Trichuris trichiura that is often used in research on experimental animals is Trichuris muris. Administration of T. muris infective eggs can significantly increase the thickness of the tunica muscularis of the colon. This thickening of the tunica muscularis intestine is associated...

Bagasse is one of the solid wastes from sugar factories which contains lignocellulose. Lignocellulose contains high levels of cellulose so that it has the potential as raw material to produce bioethanol. The crude cellulase produced by Phanerochaete chrysosporium has enzymes capable of degrading lignin,...

Capsicum sp. Has been reported as a salt-sensitive crop. Therefore, using salt-tolerant varieties will be a wise solution for maintaining crop production in coastal areas. Source of saline-tolerance controlling genes are needed to develop saline-tolerant genotypes. The objective of this study was to...

The estuary area is a semi-enclosed water body area that borders the sea but still connected to fresh water from rivers. As an area that connects between sea and rivers, the estuary is an area that threatened by human activities, including water pollution of heavy metals. Therefore, it is necessary to...

Amelogenesis imperfecta (AI) and dentinogenesis imperfecta (DGI) are hereditary dental disorders. AI is a developmental condition resulting in enamel defects of all or almost all teeth. The prevalence ranges from 1:700 to 1:14,000 for AI and from 1:6000 to 1:8000 for DGI. DGI is a collective of autosomal...

Biogas is a methane-rich gas produced from microbial digestion of waste (agricultural, sewage, and landfill) which can be used for power production. The low rate of biogas production from an anaerobic digester becomes a problem in cow manure processing increasing. Biogas production is influenced by the...

Systemic lupus erythematosus (SLE) is an autoimmune disease caused by combined genetic and environmental factors that change the normal pathways of human immunity. SLE is potentially fatal and carries significant morbidity. The SLE patient can inherit multiple predisposing genes. T The identified SLE...

Microbial utilization of agricultural biomass wastes has been widely used to synthesize certain an enzyme. We previously investigated that cellulase can be produced under solid stated fermentation based on coffee pulp waste substrate using Aspergillus sp. VTM1. Crude cellulase production was optimized...

Cellulase has been widely used in many applications of industries and is commonly produced by a cellulase-producing-fungus using production substrates from agricultural wastes biomass. In the wet processing of the coffee, up to 63% of coffee pulp rich-cellulose has been released. This potential waste...

Mirabilis jalapa is a dry tropical plant which is well grown in Indonesia. Betaxanthin active compound is present in the M. jalapa plant, which plays a role in iron metabolism. Cytotoxicity activity is one important assay for drug development. Therefore, this study aimed to determine whether ethanol...

Pectinase is a heterogeneous enzyme that hydrolyzes pectin, which is currently in high demand in the industry, so a strategy is needed to produce it economically. Aspergillus VTM4 is a mold that can grow on pomelo pulp and produce pectinase. Under solid-state fermentation, the crude pectinase was produced...

Hydrolytic Pectinase is an important enzyme and is widely used in several industries. In the previous investigation, this enzyme could be produced by Aspergillus sp. VTM5 under solid-state fermentation and the coffee pulp waste substrates were used. The specific activity of about 0.4 U/ml of pectinase...

Coffee pulp is an agro-industrial waste that is produced in huge amounts in Indonesia. The coffee pulp contains hemicellulose, with 63% of the total cellulose. In our previous study, the cellulase activity of Penicillium sp VT11 from coffee pulp waste was assayed under the solid-state fermentation (SSF)...

Cellulase can be produced by a microorganism, such as fungi. The cellulolytic microorganism used in this study was Paecilomyces lilacinus ICP1. P. lilacinus ICP1 is one of the indigenous fungi from coffee pulp that has been known to produce cellulose. Coffee pulp is expected to be able to replace the...

Coffee pulp waste contains 25.9–33.0% hemicellulose. Hemicellulolytic bacteria possess the ability to depolymerize hemicellulose by producing hemicellulase enzymes. The objective of the research is to isolate and identify the hemicellulolytic bacteria from Indonesian coffee pulp waste. Hemicellulolytic...

Breast cancer is most common cancer diagnosed in women. The urgency of developing effective therapeutic approaches is needed, both passive and active immunotherapy using vaccines. Immunoinformatics approach for epitope prediction of cancer proteins is one of promising approach in peptide vaccine development....

Cordyline fruticosa, also known to Indonesians as andong, is one of the simplest plants to grow. This plant is commonly used as an ornamental plant in yards, gardens, cemeteries, as well as a road barrier. Although it is primarily grown for ornamental purposes, the leaves of this plant empirically have...

Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production...

Brebes Regency is a bancroftian filariasis endemic area. Culex quinquiefasciatus mosquito is accused as a vector transmitting bancroftian filariasis. The eradication of disease vector that has been widely performed in Indonesia relies on the use of malathion insecticide. The use of such insecticide in...

Cardiovascular diseases are the leading cause of mortality over the world, which is approximately 17.9 million patients’ death in 2019 or 32% of all deaths worldwide. Atherosclerosis formation due to accumulated plaques in the tunica intima of vascular walls underlies the pathogenesis of cardiovascular...

Jaranan plant (Lannea coromandelica) grows massively in the buffer zone and utilization zone of Baluran National Park. This plant produces gum in branches and stems. Local people extract gum by cutting down stems as medicine for diarrhea, typhus, and eye infections treatment. The study aims to test gum...

The Aedes aegypti mosquito has been known as the primary vector in the transmission of Dengue Hemorrhagic Fever (DHF). D7 protein has been reported as a protein expressed in the salivary glands of mosquito vectors that can increase the transmission of the pathogen because of its ability to immunosuppress...

Vanda tricolor Lindl. is one of the important ornamental plants favored by many people in the world. However, this plant required many years for vegetative growth before the first flowers emerge. Since the flower is one of the most important organs in morphological identification, an alternative approach...

Erek-erek is Ijen Geopark Biosite located in an area above 1000m altitude. It is a primary tropical rain forest ecosystem in a quarter volcano which is already in the stage of climax succession because the canopy stratification is very complex. However, there is limited information on the tree vegetation...