Modifying High Sucrose Tomatoes by Genome Editing A-Review

DOI

10.2991/978-94-6463-062-6_31How to use a DOI?

Keywords

CRISPR/Cas9; Genome editing; Sucrose; Tomato

Abstract

Sucrose which is the main product of photosynthesis in plants is resulted from carbon assimilation. The way to produce more sucroses as the sink product has been done a lot recently. However, the way such as overexpression of SPS or harvest environmental engineering needed a lot of samples and a certain period of time. Genome Editing promises giant leaps forward in advancing biotechnology, agriculture, and basic research whereby targeted mutations can be introduced into a plant genome in a highly specific manner through great precision. This technology does not incorporate transient modifications that become far superior to conventional plant breeding. The recent technique of genome editing is Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) which has greatly advanced the breeding for crop improvement due to its simplicity and high efficiency over other nucleases. CRISPR/Cas9 tool contains a non-specific Cas9 nuclease and a single guide RNA leading Cas9 to the specific genomic location creating double-strand breaks and subsequent repair process creates insertion or deletion mutations. In this paper we aim to focus on the application of CRISPR/Cas9 to increase the sucrose level of tomato fruit (Solanum lycopersycum L.). We will review the editing strategy including conversion of vacuolar invertase (TIVI) into cell wall invertases (TIVI1), deletion of sucrose-induced repression of translation (SlbZIP2), and generation active sucrose phosphate synthase (SPS). Tomato cotyledon explant is being targetted with Agrobacterium infection. CRISPR/Cas9 enzyme can give novel characters in plant by increasing, decreasing and removing expression of a gene target. The golden gate cloning make addition of the characters in plants can be done by over 10 characters in a single crop. It should be no rejection of genome editing product due to the gene’s purity sequence from other organism.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Muhammad Mufarrij Fuad Ulfi
AU  - Ridlo Firmansyah
AU  - Wahyu Indra Duwi Fanata
AU  - Dibyajyoti Pramanik
AU  - Jae-Yean Kim
AU  - Sholeh Avivi
PY  - 2022
DA  - 2022/12/22
TI  - Modifying High Sucrose Tomatoes by Genome Editing A-Review
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 312
EP  - 320
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_31
DO  - 10.2991/978-94-6463-062-6_31
ID  - Ulfi2022
ER  -