Electroelution of 31 kDa Immunogenic Protein Fraction from the Salivary Gland of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

DOI

10.2991/978-94-6463-062-6_23How to use a DOI?

Keywords

Dengue; Electroelution; Immunogenic protein; Purification

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease generated by dengue virus infection. The dengue virus is transmitted to the host by mosquito vectors. The main and secondary vectors are Aedes aegypti and Aedes albopictus. Dengue virus transmission is mediated by salivary gland proteins that facilitate the blood-feeding process. Previous studies have shown that the molecular weight of 31 kDa is an immunogenic protein of Ae. aegypti belonging to the D7 family based on the result of Mass Spectrometry. This immunogenic protein can be used for the development of vector-based dengue vaccines. The purpose of this study is to purify the 31 kDa protein fraction from the salivary gland of Ae. aegypti and Ae. albopictus using the electroelution method. The study is conducted by collecting the salivary glands of Ae. aegypti and Ae. albopictus, SDS-PAGE, electroelution using the Bio-Rad 422 electroeluter, Dot Blot, and Western Blot. Both protein samples resulting from electroelution confirmed with a single band appeared in SDS-PAGE. The optimal conditions for electroelution are 3 h running time, 100 voltage, volume ±1 mL, and the concentration obtained are 1.789 mg/mL (Ae. aegypti) and 1.81 mg/mL (Ae. albopictus). These results are supported by the other result of the dark dots shown in Dot Blot and a single band of 31 kDa shown in Western Blot when it reacted with the serum of dengue patients, endemic healthy people, and neonates. These results indicate that the purified 31 kDa immunogenic protein fraction can be recognized by specific antibodies.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ilma Zakiyyah
AU  - Linda Dwi Santika
AU  - Syubbanul Wathon
AU  - Kartika Senjarini
AU  - Rike Oktarianti
PY  - 2022
DA  - 2022/12/22
TI  - Electroelution of 31 kDa Immunogenic Protein Fraction from the Salivary Gland of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 234
EP  - 248
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_23
DO  - 10.2991/978-94-6463-062-6_23
ID  - Zakiyyah2022
ER  -