Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation
- DOI
- 10.2991/978-94-6463-062-6_56How to use a DOI?
- Keywords
- CMCase; Actinomycetes; Nicotiana tabacum L.; Submerged fermentation
- Abstract
Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production by three actinomycetes with the best CMCase activity index using tobacco stalk as substrate through submerged fermentation, and morphology identification actinomycetes with the highest CMCase activity. A total of 71 actinomycetes have been isolated from the rhizosphere of tobacco plant. In this study, the ability of actinomycetes to produce cellulase through submerged fermentation using tobacco stalk substrate powder. There were about 71 actinomycetes isolated and all of them have cellulolytic activities. Based on the screening of cellulolytic activity on CMC (carboxymethyl cellulose) agar plate, the enzyme activity index of ATG 38 isolate was higher than others, with 2.50 ± 0.26 respectively. The cellulase production using tobacco stalk as substrate through submerged fermentation was optimal showing that CMCase production of actinomycete ATG 38 isolates maximal after four days incubation with 0,40 mU/mL enzyme activity. Microscopic morphological observations showed that actinomycete ATG 38 isolate belongs to the genus Streptomyces.
- Copyright
- © 2023 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Esti Utarti AU - Annisa’ul Jannah AU - Sattya Arimurti PY - 2022 DA - 2022/12/22 TI - Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation BT - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021) PB - Atlantis Press SP - 550 EP - 559 SN - 2468-5747 UR - https://doi.org/10.2991/978-94-6463-062-6_56 DO - 10.2991/978-94-6463-062-6_56 ID - Utarti2022 ER -