Production and Partial Purification of Cellulase from Aspergillus sp. VT12 by Solid-State Fermentation Using Coffee Pulp

DOI

10.2991/978-94-6463-062-6_47How to use a DOI?

Keywords

Aspergillus sp. VT12; Cellulase; Coffee pulp; Solid-state fermentation; Partial purification

Abstract

Cellulase has been widely used in many applications of industries and is commonly produced by a cellulase-producing-fungus using production substrates from agricultural wastes biomass. In the wet processing of the coffee, up to 63% of coffee pulp rich-cellulose has been released. This potential waste allows being used as a substrate for enzyme production. This study aims to produce cellulase using coffee pulp by Solid-State Fermentation from isolates of Aspergillus sp. VT12. A solid-state fermentation medium of coffee pulp was inoculated with Aspergillus sp. VT12 and incubated at 37 ℃ for 5 days, the culture produce a maximum cellulase activity of 0.52 U/ml against 0.5% carboxymethyl cellulose substrate. For the purification step, the crude cellulase obtained was dialyzed on a cellulose tube membrane 12–14 kDa and then loaded into open column anion exchanger DEAE Cellulose DE-52 chromatography with NaCl gradient 0–0.6M used. In this step, the activity of purified cellulase obtained was 1.00 U/ml with a yield of 51.86%. For all steps of dialysis and cellulase measurement activity, including purification steps, the 20 mM acetate buffer pH 5 was used. This research revealed that the potential coffee pulp waste can be used as a substrate for cellulase production. However, in an economical view, production efficiency still needs to be optimized and the purity of the enzyme as well.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Farah Salma Elida
AU  - Dwi Fajarwati Ramadhani
AU  - Rudju Winarsa
AU  - Hidayat Teguh Wiyono
AU  - Siswoyo
AU  - Kahar Muzakhar
PY  - 2022
DA  - 2022/12/22
TI  - Production and Partial Purification of Cellulase from Aspergillus sp. VT12 by Solid-State Fermentation Using Coffee Pulp
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 467
EP  - 474
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_47
DO  - 10.2991/978-94-6463-062-6_47
ID  - Elida2022
ER  -