In Vitro Cytotoxicity of Gallic Acid Derivatives (Alkyl gallates) Against Breast MCF-7 Cancer Cells

DOI

10.2991/978-94-6463-062-6_26How to use a DOI?

Keywords

Gallic acid derivative; Alkyl gallate; Cytotoxicity; MCF-7 cell

Abstract

Breast cancer is the most common cancer in women. Based on WHO data in 2011, it is estimated that more than 508,000 women worldwide died of breast cancer. In Indonesia, the prevalence of breast cancer is 26.5% of all cancer cases, in which become the second frequent cancer. To date, the treatments for breast cancer are surgery, radiation therapy, chemoradiation, or combination therapy. However, the high rate of complications and side effects caused led to the need for the development of other therapies. Gallic acid is known to have potential anticancer effects. Structure modification on gallic acid by esterification of carboxyl group into alkyl gallate derivatives would change the hydrophobicity, as well as change its solubility and cytotoxicity against cancer cells. In this research, ten gallic acid derivatives (alkyl gallates) have been synthesized from the esterification of gallic acid and corresponding alkyl alcohol. Purification of the derivatives were conducted by flash column chromatography on silica gel by using the mixture of chloroform and methanol as mobile phase. Cytotoxic activities of gallic acid and its synthesized alkyl gallates against breast MCF-7 cancer cells were evaluated by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The data were analyzed by linear regression method to generate IC₅₀ value. Among alkyl gallate derivatives, isoamyl gallate, heptyl gallate and octyl gallate showed a moderate cytotoxicity on breast MCF-7 cancer cells with IC₅₀ values of 58.11; 25.94 and 42.34 μg/mL, respectively. Thus, isoamyl gallate, heptyl gallate and octyl gallate should be further developed as potent and promising agents for the treatment and therapy of breast cancer.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ade Arsianti
AU  - Maya Dorothea
AU  - Naura Syafira
AU  - Ananda Tony
AU  - Anton Bahtiar
PY  - 2022
DA  - 2022/12/22
TI  - In Vitro Cytotoxicity of Gallic Acid Derivatives (Alkyl gallates) Against Breast MCF-7 Cancer Cells
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 266
EP  - 276
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_26
DO  - 10.2991/978-94-6463-062-6_26
ID  - Arsianti2022
ER  -