Isolation and Identification of Biogas-Producing Methanogenic Bacteria from Cow Manure

DOI

10.2991/978-94-6463-062-6_44How to use a DOI?

Keywords

Biogas production; Cow manure; Identification; Isolation; Methanogenic bacteria

Abstract

Biogas is a methane-rich gas produced from microbial digestion of waste (agricultural, sewage, and landfill) which can be used for power production. The low rate of biogas production from an anaerobic digester becomes a problem in cow manure processing increasing. Biogas production is influenced by the biomass of methanogenic bacteria in the conversion of organic matter contained in a digester, so other methanogenic bacteria are needed to accelerate the rate of biogas production, namely methanogenic bacteria from the anaerobic digester of cow manure. Bacterial isolation was carried out by isolating samples of the anaerobic digester of cow manure in fluid thioglycolate media using the pour plate method in a vial tube. Those samples were incubated at 37 ℃ in an anaerobic chamber where after isolation, the bacteria were identified through several biochemical tests. Based on research conducted, a single colony of methanogenic bacteria is gram negative bacterium where the results of the isolates show that the bacteria of the Methanobacterium genus. The highest biogas production was obtained from the addition of 15%v/v of bacterial isolate. It can be seen from the volume of biogas produced with the volume 40 mL for 14 days of the fermentation process.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Grace Roma Artha Samosir
AU  - Ellyas Alga Nainggolan
AU  - Meiyer Marthen Kinda
AU  - Dedy Anwar
PY  - 2022
DA  - 2022/12/22
TI  - Isolation and Identification of Biogas-Producing Methanogenic Bacteria from Cow Manure
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 444
EP  - 450
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_44
DO  - 10.2991/978-94-6463-062-6_44
ID  - Samosir2022
ER  -