An Extracellular Cellulase Production Under Solid-State Fermentation of Coffee Pulp Waste by Aspergillus sp. VTM1 and Its Purification

DOI

10.2991/978-94-6463-062-6_46How to use a DOI?

Keywords

Aspergillus sp. VTM1; Cellulase; Coffee pulp; Purification; Solid-state fermentation

Abstract

Microbial utilization of agricultural biomass wastes has been widely used to synthesize certain an enzyme. We previously investigated that cellulase can be produced under solid stated fermentation based on coffee pulp waste substrate using Aspergillus sp. VTM1. Crude cellulase production was optimized for a week of incubation at 30 ℃, the crude was harvested daily with distilled water containing 1% NaCl and 0.1% NaN3. To measure cellulase activity, the reducing sugar Somogy-Nelson method was used. In the next step, crude cellulase was dialyzed using a 45µm cellulose membrane tube 12–14 kDa against acetate buffer pH 5. Further analysis found that the maximum activity of cellulase production was obtained after inoculating 10⁸ spores/ml of Aspergillus sp. VTM1 to the 5g of sterile coffee pulp (67% humidity) at 30 ℃ for 96 h. Large-scale production of cellulase crude extract results in an activity of 0.70 U/ml with a total activity of 121.1 U/ml. After the dialysis, the enzyme activity (0.81 U/ml) and specific activity (0.01 U/mg) were increased compared to the crude extract. On an anion exchanger using DEAE cellulose DE-52, the specific activity was 0.1 U/mg, increased 25.03 times with a yield of 36,82%. The findings of this study lead to a green strategy for cellulase production under solid-state fermentation by Aspergillus sp. VTM1 and its purification.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ramdhan Putrasetya
AU  - Reni Rusdianti
AU  - Viara Septaninda Sugianto
AU  - Rudju Winarsa
AU  - Siswoyo
AU  - Kahar Muzakhar
PY  - 2022
DA  - 2022/12/22
TI  - An Extracellular Cellulase Production Under Solid-State Fermentation of Coffee Pulp Waste by Aspergillus sp. VTM1 and Its Purification
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 460
EP  - 466
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_46
DO  - 10.2991/978-94-6463-062-6_46
ID  - Putrasetya2022
ER  -