The Cytotoxicity Effect of Ethanol Extract and Alkaloid Fraction of Mirabilis jalapa Leaves in Hepatocarcinoma Cell Line

DOI

10.2991/978-94-6463-062-6_48How to use a DOI?

Keywords

Betaxanthin; Cytotoxicity Effect; HepG2 Cells; Mirabilis jalapa leaves

Abstract

Mirabilis jalapa is a dry tropical plant which is well grown in Indonesia. Betaxanthin active compound is present in the M. jalapa plant, which plays a role in iron metabolism. Cytotoxicity activity is one important assay for drug development. Therefore, this study aimed to determine whether ethanol extract and alkaloid fraction of M. jalapa leaves had a cytotoxic effect or not in hepatocarcinoma (HepG2) cell line. M. jalapa plants were cultivated in Merapi Farma, Yogyakarta. Extraction and fractionation of M. jalapa leaves were performed in the Pharmacy Laboratory, Surakarta. HepG2 cell line was subcultured in a complete culture media. Confluent cells were then treated with extract and fraction of M. jalapa leaves for 24 h. To determine the toxic dose of extract and fraction of M. jalapa leaves, we used tetrazolium bromide (MTT) and Resazurin assays, which was indicated as 50% inhibitory concentration (IC₅₀). In the MTT assay, IC₅₀ of ethanol extract was 51.69 ppm, higher than the IC₅₀ of alkaloid fraction (47.79 ppm). In comparison with the Resazurin assay, the IC₅₀ both ethanol and alkaloid fraction were higher than that of the MTT assay (135.87 and 149.08 ppm respectively). Furthermore, these toxic doses were still safe for HepG2 cells (>30 ppm). Ethanol extract and alkaloid fraction of M. jalapa leaves have low cytotoxic effect in HepG2 cell line. The next research is required to evaluate the effect of extract and fraction of M. jalapa leaves on iron metabolism in HepG2 cells.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Yuliana Heri Suselo
AU  - Dono Indarto
AU  - Brian Wasita
AU  - Hartono
PY  - 2022
DA  - 2022/12/22
TI  - The Cytotoxicity Effect of Ethanol Extract and Alkaloid Fraction of Mirabilis jalapa Leaves in Hepatocarcinoma Cell Line
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 475
EP  - 481
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_48
DO  - 10.2991/978-94-6463-062-6_48
ID  - Suselo2022
ER  -