Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Authors
Azizah1, Atim Ainul Hidayah1, Rosa Amelia1, Hidayat Teguh Wiyono1, Siswoyo2, Kahar Muzakhar1, *
1Department of Biology, Faculty of Mathematics and Natural Sciences, Jember University, Jember, Indonesia
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jember University, Jember, Indonesia
*Corresponding author. Email: kaharmzk@unej.ac.id
Corresponding Author
Kahar Muzakhar
Available Online 22 December 2022.
DOI
10.2991/978-94-6463-062-6_50How to use a DOI?
Keywords
Coffee pulp; Pectinase; Production; Purification
Abstract

Hydrolytic Pectinase is an important enzyme and is widely used in several industries. In the previous investigation, this enzyme could be produced by Aspergillus sp. VTM5 under solid-state fermentation and the coffee pulp waste substrates were used. The specific activity of about 0.4 U/ml of pectinase was detected when 40gr coffee pulp water-saturated inoculated with 1.2x107 spores of Aspergillus sp. VTM5 was incubated at 30 ℃ for 168 h, and without any nutrients added. Against 0.5% alkaline extract of pomelo pulp substrate in 20 Mm acetate buffer pH 5, pectinase activity increases up to 1.03 U/ml, and the purity 3.5 times, after dialyzed using 12–14 kDa, pores cellulose membrane tube higher than previously step. Open column purification using Fractogel EMD SO3-(S) cation exchange chromatography, pectinase was eluted at 0.45 M NaCl. The pectinase activity significantly increased to 0.295 U/ml with 12.37-fold and 17.04% yield. Based on these results, it can be proved that coffee pulp waste is suitable as a substrate for pectinase production by Aspergillus sp. VTM5. Further research to analyze the properties and characterize the stability of pectinase against temperature, pH, the effect of heavy metals, and molecular analysis is needed.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 December 2022
ISBN
978-94-6463-062-6
ISSN
2468-5747
DOI
10.2991/978-94-6463-062-6_50How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Azizah
AU  - Atim Ainul Hidayah
AU  - Rosa Amelia
AU  - Hidayat Teguh Wiyono
AU  - Siswoyo
AU  - Kahar Muzakhar
PY  - 2022
DA  - 2022/12/22
TI  - Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 492
EP  - 500
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_50
DO  - 10.2991/978-94-6463-062-6_50
ID  - 2022
ER  -