Amylase Production by Rhizopus oryzae Using Solid State Fermentation with Cassava Solid Waste as Substrate

DOI

10.2991/978-94-6463-062-6_25How to use a DOI?

Keywords

Amylase; Cassava solid waste; Central composite design; Rhizopus oryzae; Solid state fermentation

Abstract

Amylase is an enzyme that is used in various industries such as food, paper, and textile industries. Amylase can be produced by microorganisms through fermentation. Rhizopus oryzae is one of microorganisms that can produce amylase through fermentation. Tapioca solid waste has a good potential as a carbon source for microorganisms for producing amylase since it contains high carbohydrate. Tapioca solid waste produced in Toba Regency, North Sumatera, reaches 126 tonnes per day, and the use is limited as compost and forage. It is possible to use tapioca solid waste as a substrate for amylase production thus providing added value to it. Fermentation was carried out for 7 days at 35 ℃. After extraction, amylase was partially purified by ammonium sulfate at 40% and 60% saturation level followed by dialysis. Amylase activity was determined spectrophotometrically using dinitrosalicylic acid (DNS) method. Partially purified amylase in 60% ammonium sulfate fraction has the highest activity of 47.6278 U/mL and the highest yield of 97.51% with purity level of 0.653 times. Optimum condition of amylase activity was at pH 6.5 and temperature of 60 ℃ with 30.1797 U/mL activity. The ability of amylase in hydrolyzing starch was analyzed in various time duration (3, 6, 9, and 12 h) and various ratio of enzyme against substrate (1: 2, 1:5, and 1:10). The highest hydrolysis activity of amylase was 44.3778 U/mL, obtained from the ratio 1:10 of enzyme against substrate and 12 h of hydrolysis time.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Merry Meryam Martgrita
AU  - Roga Florida Kembaren
AU  - Herti Novalia Hutapea
AU  - Ivana Sitepu
AU  - Evy Enjelina Simanjuntak
PY  - 2022
DA  - 2022/12/22
TI  - Amylase Production by Rhizopus oryzae Using Solid State Fermentation with Cassava Solid Waste as Substrate
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 257
EP  - 265
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_25
DO  - 10.2991/978-94-6463-062-6_25
ID  - Martgrita2022
ER  -