The feasibility of evaluation thermal damage in real time by Visible-Near Infrared (V-NIR) spectroscopy is investigated. Thermal damage are produced in fresh porcine livers in vitro by laser (808nm, 5W). The V-NIR spectra (330-1100 nm) at distances of 4, 8 and 12 mm from the center of the damage are...

Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study...

The fragment encoding aromatase (CYP19) with 45 N-terminal amino acids cut-off was cloned into pPIC9K vector and transformed to the yeast Pichia pastoris. The multi-copy recombinant strain was selected for the production of recombinant aromatase. The catalytic bioactivity of secreted enzyme was further...

Oridonin, the main pharmacological active substance isolated from Rabdosia Rubescens, has been found to show anti-inflammatory, pro-apoptotic, neurological effects, and excellent anticancer activities. However, the clinical application of oridonin is still limited by its poor solubility. The special...

Objective: This study aims to compare the cytotoxicity and adhesion difference between oxalate monohydrate (COM) and dehydrate (COD) crystals and African green monkey kidney epithelial cells (Vero). Methods: Through the detection of cell viability, lactate dehydrogenase (LDH) release amount, expression...

Objective To research intracranial pressure change rules of craniocerebral injury through experimentation on animals. Method According to the difference of the hit parts, the animals are divided into three groups to collect the intracranial pressure changes through data collection system andpressure...

The acupuncture signal in the uplink conduction, one side through the spinal cord segmental relationship affect the adjacent segments dominated by the skin, visceral activity and adjacent segments of the pain in the introduction; more often up to reach the brain stem, Forebrain and other parts, through...

Objective: To analyze the methods and characteristics of animal model of oral ulcer and the characteristics of Chinese and Western medicine oral ulcer disease anastomosis. Methods: To summarize the characteristics of animal models of oral ulcers, modeling methods, analysis of oral ulcer animal model...

Characterization can reflect objectivity and represent objective things. With the development of pharmacology of Chinese Medicine in recent years and the deepening of animal model research in Chinese medicine, how to accurately and quickly copy the successful model animals according to the characterization...

Objective: To explore the therapeutic principles of modern Chinese medicine for chronic prostatitis and the application of TCM in CNP. Procedures and Methods: Analysised the relevant information and literature, to explore the mechanism of Chinese medicine for the treatment of prostatitis, sum up TCM...

With the study of anti-allergy medicine, it proves traditional Chinese medicine has anti-allergic effects and several other functions, such as protecting and stabilizing the target cell membrane (reduce or prevent degranulation, release the allergy mediators, increase the intracellular cAMP levels),...

From the reform and opening up in 1979 to the year of twenty-first Century, China's economy has been growing rapidly,and great changes have taken place in people's traditional eating habits. Gradually westernized modern diet has become one of the main reasons ,which leading the incidence of many chronic...

This paper summarizes the research achievements of predecessors in the field of toxicology of Chinese medicine, discusses the new understanding of the toxicity of Chinese medicine from the aspects of the toxicity of Chinese medicine, the application of the toxicity of Chinese medicine and the trend of...

In this paper, the growth law of the phosphate and calcium layer in different voltage process was studied. The surface morphology of the film was observed by SEM, and then the distribution of phosphorus and calcium in the film was analyzed. The results show that the higher the voltage, the more uniform...

This study is to uncover VHD-related important biological features for understanding molecular of this disease. The system analysis strategy is applied to generate the first VHD-related FFL network. Then, the network topology is mapped into the biological space to refine the biological features of VHD....

Calcium, as the second messenger inside plants, is extensively involved in plant response and transduction of various stress signals. Plants shall generate calcium signals of specificity under stress, and this shall give a rise to a series of intracellular physiological and biochemical responses. Calcium...

In this paper, a set of experiments scanning real potential variations inside cavities under current control were employed to characterize local electrochemical information. The limiting diffusion current and the critical currents sustaining the supersaturation of dissolved gas and completed oxide film-formation...

Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a...

Objective: Analysis of alopecia areata animal model production methods, characteristics and characteristics of Chinese and Western alopecia areata anastomosis. Methods: To summarize the characteristics of the alopecia areata animal model, according to alopecia areata diagnosis and clinical characteristics...

In recent years, The situation that China's fog and thick haze pollution is very grim, which has threatened people's health and daily life work. fog and thick haze is a meteorological phenomenon, which is the essence of human activities caused by fine particulate pollution. With the increase in the severity...

In this report, we have identified a novel serine carboxypeptidase, named HoSCP, from the midgut of a destructive agricultural and landscape pest, Holotrichia oblita. HoSCP cDNA gene was cloned by immunoscreening method. Sequence analysis showed about 49kDa HoSCP consists of 457 amino acid residues with...

In this work, we used PAM fluorometry to examine photosynthetic characteristics of marestail (Hippuris vulgaris) across a water temperature gradient in three lakes. Three lakes were studied across a gradient in water temperature, with low water temperature conditions in Grass Lake and Arrow Bamboo Lake,...

In this paper, a bioink composed of multiple native materials with tyrosine micro-particle reinforcement for 3D bioprinting of skin scaffolds was developed. The rheological property of the developed bioink was optimized for 3D Bioprinting process. The results showed that Tyrosine micro-particle reinforcement...

Objective: Based on the characteristics of clinical symptoms of pruritus, analyze the relevant animal model, and then propose the evaluation and improvement. Method: According to the clinical diagnostic criteria of pruritus in Chinese and Western medicine, summarize the characteristics and modeling methods...

Objectives: To investigate the characteristics and mechanism of flower traditional Chinese medicine for preventing and treating cerebral ischemia. methods: To summarize active components of flower traditional Chinese medicine on cerebral ischemia model and the characteristics of prevention and treatment...

Purpose: To study the injury difference of the un-aggregated and aggregated calcium oxalate monohydrate (COM) on human kidney proximal tubular epithelial (HKC) cells, and to discuss the molecular and cellular mechanism of kidney stone formation. Methods: The cell injure by crystals was evaluated by measuring...

Purpose: The oxidative damage of hydrogen peroxide (H2O2) on human kidney proximal tubular epithelial cell (HKC) and the nucleation and growth of calcium oxalate (CaOx) crystals modulated by normal and injured cells were investigated. Methods: The method of CCK-8 was used to detect the proliferating...

Based on the current research value of biomass and terpenoids, in this paper, the terpenoids contented in the different organizational of the camphor tree were analyzed by SDE extract method and GC-MS analysis method. The results showed that the contents of terpenoids contained in camphor leaves were...

1, 3-propanediol (1,3-PD) is an important chemical material, which is wildly used in many fields. Clostridium butyricumis key strain which can transform glycerol into 1,3-PD. In this study, a new strain producing 1,3-PD was isolated from soil. On the basis of morphology, biochemical-physiological characteristics,...

In this study the microbial composition in the starter (Daqu) of Chinese Gujing Tribute liquor was determined by employing near full length ribosome gene library sequencing approach. Gujing Tribute Daqu has a distinct microbial community structure by comparison with several other important types of Chinese...

Strong-aroma type liquor occupies over 70% of Chinese liquor market, but the namelist of key microbes responsible for liquor flavor is still vague. Lots of data indicate that the interface between the bottom pit mud and Zaopei (fermented grains) plays an important role for aroma molecule production,...

Objective: To determine the antitumor activity of five benzamide derivatives with similar structure in A549 tumor cells. Methods: Cytotoxicity of the compounds in A549 tumor cells were monitored through an xCELLigence Real-Time Cell Analysis System. Results: Four benzamide analogs inhibited the growth...

Objective: we investigated the function of Pingyan capsule on antitumor and immune regulation in Lewis lung cancer tumor-bearing mice. Methods: mice were randomly divided into normal control group, model group, positive control group, high, medium and low dose Pingyan capsule groups. Lewis lung carcinoma...

Many proteins function execution depends on the process of protein and ligand interact with each other. The identification of ligand binding residues is important for the research of the protein function. The 4442 protein chains with

In this study, the cardinal and purple peanut testa were used as the raw materials for extracting polyphenol substances with ultrasonic and freeze-drying. Then the bacteriostasis and antifungal activities of the extracts were investigated by the methods of disc diffussion and equivalent against the Benzylpenicillin...

Phytoremediation is becoming a cost-effective technology for the in-situ clean up of sites polluted with hydrophobic organic contaminants (HOCs). The major factors limiting phytoremediation are the mass transfer, rate of plant uptake, and microbial biodegradation of HOCs. To evaluate the potential of...

Aqueous two-phase system (ATPS) combined with ultrasonic technology was employed to extract polysaccharide from hawthorn leaves in this study. The partition behaviors in different ATPS were investigated first, polyethylene glycol (PEG) 11.5% and (NH4)2SO4 14% were selected as the optimum solvent for...

Fucoidan is a complex sulfated polysaccharide mainly found in brown seaweed. It has been used in a wide range of applicationsacross different industries such as food additives, dietary supplements and pharmaceuticals. Studies have shown that fucoidan has numerous biological activities including anti-cancer,...

To observationthe relationship between spinal inhibition of DNA methylation in electroacupuncture (EA) analgesia, and spinal cord Dnmt3amRNA expression. EA and inhibitors of 5-AZA Dnmt can improve CCI animals hyperalgesia state and decreased Dnmt3amRNA expression. DNA methylation is closely related to...

Objective: Analysis anastomosis of the commonly used animal models and clinical features of chronic skin ulcer disease, put forward to recommendations for model refinement. Method: Summarize the characteristics of existing chronic skin ulcer model and molding method, according to traditional Chinese...

To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis(Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla chinesis(Bge.) Regel., collected in Jilin Province, amplified the ITS and trnL-F region using...

Objective: to discuss the effectiveness and safety of the ureteral stone in complicated ureteroscope of ureteroscope. Methods: in this study, 47 cases of ureteral calculi were treated by the ureteroscope of the ureteroscope, which was collected from February 2015 to February 2017. Results: in 47 cases,...

Objective To compare the efficacy and complications between bipolar plasmakinetic resection of prostate and bipolar plasmakineticenucleation of prostate for benign prostatic hyperplasia(BPH). Methods 303 cases of BPH were divided into 2 groups, 143 cases aged (73ñ13.4) yearswith prostate volume of (76.5ñ22.5)ml...

Objective: to study the electronic soft ureter mirror joint qin laser lithotripsy in the treatment of renal calyx calculi under safety and effectiveness.Methods: our hospital in March 2016 - March 2017 electronic soft ureter mirror qin laser lithotripsy under 40 patients under the treatment of renal...

Long range surface plasmon resonance (LRSPR) was used to improve the propagation lengths, electric field strengths, and the sharpness of angular resonance curves compared with the conventional surface plasmon resonance (CSPR). LRSPR sensor chip was designed with buffer layer whose refractive indices...

Performance monitoring tasks generate response-locked event-related potentials (ERPs), generated from anterior cingulate cortex (ACC) region of the medial prefrontal cortex. However, the quantitative relationship between mental fatigue and error monitoring has not been closely investigated, which is...

B-vitamins can be used as cofactors in the synthesis process of L-threonine. Concentration of the cofactor can effectively change the activity of specific enzymes and reallocate the distribution of carbon substances in the cells. Theeffects of B-vitamins, including calcium pantothenate (VB5), cobalamin...

One mitochondrial minisatellite locus was used to study cytoplasmic diversity in leaf beet lines SK-5 population from Chongqing, China. The analysis of one mitochondrial minisatellite locus (TR2) was conducted using variable number of tandem repeats (VNTRs) molecular markers method within 50 single plants...

Purposes: Study the changes of cardiac and brain function of exercise related sudden death in rats under over fatigue state and explore the causes of sudden death preliminarily. Methods: Totally 72 male SD rats.7 rats were randomly selected as negative control group. The others were trained continuously...

The purpose of this study was to investigate the exercise intensity of endurance training of altitude training under the plain and plateau by means of the fixed maximal oxygen expenditure. Eight middle and long distance runners voluntarily participated in this study, the ergmeter bike and telemetry cardiopulmonary...

To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA...

For human beings, the morphological features of cells are closely related to human health thus research into the technique of cell imaging is very important. The optical phase imaging technique is one of the most useful methods because the cell is a phase object. More than one phase images should be...

By immobilizing glucose oxidase (GOD) on a nanocomposite containing hydroxyl fullerenes (C60-OH) and TiO2 nanoparticles, a new type biosensor was prepared for glucose detection. The cyclic voltammograms (CVs) of GOD on the nanocomposite modified glassy carbon electrode (GCE) was quasi-reversible. The...

A novel chitin deacetylase (CDA), HcCDA4, was identified from the American white moth, Hyphantria cunea. The full-length cDNA sequence of HcCDA4 was identified,and the cDNA is 1494bp in length. The HcCDA4 was shown as a 56 kDa protein in E. coli BL21 by SDS-PAGE analysis and the specific antibodies reacting...

A Hydroponic culture was conducted to determine the effect of organic acids and metal ions on plant growth, camptothecin concentrations and yield of Camptotheca acuminata, in an effort to increase the camptothecin yield by artificial cultivation. For maximal camptothecin production, increase of maleic...

Purpose: To research the states and feasibility of stem cells for neonatal hypoxic ischemic encephalopathy (HIE). Methods: In this study, "newborn, hypoxic ischemic encephalopathy, pathogenesis, stem cell transplantation" as the key words, which are searched the papers published in CNKI database and...

Type 1 diabetes (T1D) is a complex autoimmune disease characterized by T cell-mediated destruction of pancreatic cells, which is resulted from the interaction of genetic and environmental factors. Twin-study is one of the most important methods to identify the genetic basis of complex diseases or traits...

[Purposes] To explore the correlation between mRNA expression of NOB1 gene in tissues from ovarian cancer, normal ovary, benign ovarian tumor. [Methods] 50 samples of ovarian cancer as the experimental group, 20 samples of normal ovarian tissues and 50 samples of benign ovarian tumor as control groups....

[Purposes] To investigate the expression of microRNA-145 on invasion and metastasis of cervical cancer cells. [Methods] Analysis of the relationship between high expression of microRNA-145 on invasion and metastasis of cervical cancer cells by Transwell invasion assay and Wound scratch assay. [Results]...

The aim of the experiment was to study the histological reaction of orthodontic force on the periodontal tissues regenerated by nano biphasic calcium phosphate (NBCP) bioceramics in beagle dogs. Two third incisors were randomly selected and operated as experimental group. The contralateral third incisors...

Interleukin 1 (IL1 ) is a potential target for IBD treatment. IL1 receptor antagonist (IL1RA) can blocks the biologic activity of IL1 by competitively inhibiting IL1 binding to the IL1R, so IL1RA is suggested to have possible therapeutic applications in treatment of IBD. By using a food-grade recombinant...

With the development of aging society, the number of hemiplegia patients grows rapidly and over 75% of survivors suffering hemiplegia need rehabilitation training. Traditional training methods need one or more physical therapists to server one patient at the same time. In order to reduce the workload...

Thisÿpaperÿpresents a functional electrical stimulation system to mimic the EMG characterization of patient healthy limb to exercise his/her affected limb. Firstly, the existing problems of functional electrical stimulation system are analyzed. Generally, the existing electrical stimulation systems suffer...

A real-time fluorescence detection biomedical miniaturized system with a disposable microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. The miniaturized system was developed consisting of a mini heating plate, a temperature sensor, a temperature controller and...

Biological medicine is listed on the country's important development strategy planning in the United States, the European Union, Japan and other major developed countries and some developing countries. Through analysis of Chinese current situation of the development of biological medicine and compared...

Patients will fall asleep under anesthesiology in most cases, so they will lose their consciousness to protect themselves. Therefore, the anesthetists carry on more duties to protect the patients' privacy.

Astaxanthin is a carotenoid with strong antioxidant activity. The function of astaxanthin has been associated with a number of health-promoting benefits. This paper discusses the beneficial effects of astaxanthinin the prevention and treatment of human diseases such as cardiovascular diseases, cancer,...

Pharmacological mechanisms of Chinese herbal prescription are complicate since it contains heterogeneous components. These mechanisms are hardly understood by traditional methodology in the mass. Here we use network pharmacology approaches to explore mechanisms of JinChaiKangBingDu Capsule (JCKBD) against...

Objective:To summarize the clinical application of ureter soft mirror. Methods:The treatment of urinary calculi includes ESWL, percutaneous nephrolithotomy, ureter soft mirror and so on , The treatment of urinary calculi with ureter soft mirror is becoming more and more widespread, and the indications...

Protein crystallography is a very critical process that limits the development of pharmaceutical engineering. Microfluidic technology has provided an opportunity for protein crystallization. In this paper, a flow-free droplet-based device is presented for producing highly controlled crystals and fabrication...

The microstructure, hydrophobicity, adhesion and chemical composition of the butterfly and moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter, and a Fourier transform infrared spectrometer (FT-IR). Using ground calcium carbonate (heavy CaCO3) as contaminating...

The microstructure, wettability and chemical composition of butterfly and locust wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter and a Fourier transform infrared spectrometer (FT-IR). The hydrophobicity models were established on the basis of Cassie equation....

Objective: Analysis common animal models and vitiligo clinical symptoms anastomotic situation, put forward recommendations of improving the model. Methods: By summarizing the characteristics and modeling methods of vitiligo model and according to the clinical diagnostic criteria of vitiligo on TCM and...

Amide tautomer imidic acid were exit, which proved by NMR spectra characterization. The protein BSA was test by 1H NMR in different pH values solutions, to show the different amide/imidic acid structures exit in BSA. The amide/imidic-acid unit with H proton communication might be the biological protein...

In acidic medium, Fe (III) can be reduced to Fe (II) by hydrosulfuryl (-SH) in acetylcysteine molecule, and then using sulfosalicylic acid as chromogenic reagent of Fe (III) to determinate the acetylcysteine indirectly by discoloration spectrophotometry.An accurate fast spectrophotometric method for...

This paper presents a model of detection age which quantifies the appearance changes of human skin at different stages of aging without traditional skeleton. We provide a novel method to extract age-related primitive features from skin surface images obtained by a digital camera, including skin surface...

Dementia is the most common onset of Alzheimer's disease (AD). The main neuropathological characteristics of AD are extracellular amyloid plaques and intracellular accumulation of hyperphosphorylated Tau protein. Because of the complexity of the pathogenesis, the most treatment options are not effective...

Objective: To make intrinsic biological distinctions between qi deficiency pattern and qi stagnation pattern with genes related to NEI. Methods: Establish a data dictionary of NEI-related genes and a keyword lexicon of qi deficiency pattern and qi stagnation pattern. Then retrieve relevant literature...

To develop a rapid molecular biological method for the detection of equine infectious anemia virus (EIAV), a set of 4 primers were designed according to the conserved sequence fragment of the gag non-structure protein gene of EIAV published in GenBank, and the reverse transcriptase loop-mediated isothermal...

The cardiac SPECT is a good study because of the large clinical demand and the need for improved image quality. SPECT imaging usually suffers from poor spatial resolution and high statistical noise due to patient radiation safety concerns. Scientists proposed some high count sensitivity cardiac SPECT...

(Aim) Current cerebral microbleed detection methods are too complicated, and difficult to train. (Method) We enrolled 10 subjects diagnosed as cerebral microbleed.Our method combined wavelet entropy and naive Bayes classifier. (Results) The simulation results over 10 times of 10-fold cross validation...

It has been demonstrated that sleep has a great relationship with humans' health and activities. Sleep staging is an important method to analysis sleep. Lots of methods have been adopted for automatic sleep staging. Some of the results are good and some of them are poor. For the poor, the sleep spindle...

Beam former, as a class of Magnetoencephalography (MEG) source imaging methods, is widely used. However, it has difficulty in locating weak electromagnetic sources within brain. To solve this problem, this paper attempts to develop a method for MEG weak Source Imaging. Firstly classical beam former is...

Objective: To explore the effects of jinjiang granules on immunologic function and to provide evidence for jinjiang granules as the application of clinical medicine in the initial stage of cold, through the observation of the effects of jinjiang granules on immunologic function of low immune mouse model...

Response surface methodology (RSM) was used to optimize a protective agent for enhancing the cell viability of Staphylococcus aureus during freeze-drying. Using a previous Plackett-Burman design, it was found that sodium glutamate, trehalose, L-cysteine hydrochloride and bovine serum albumin were the...

This paper proposes a liver vessel segmentation algorithm based on fuzzy enhancement and Hessian matrix. First, a method with two steps based on the theory of fuzzy enhancement is proposed to enhance the liver image. The first enhancement step is to make the local changes in the image more obvious on...

The paper explored risk factors for uteroplacental apoplexy complicating placental abruption, the condition's clinical characteristics, and maternal and fetal outcomes.62 pregnancies of placental abruption were collected from Second Hospital Jilin University during Jan. 2007-Dec. 2012.According to uteroplacental...

By micro-arc oxidation technology, the TA2 titanium metal surface modification, by scanning electron microscopy (SEM) experiments, the effects of different voltage parameters on film thickness and surface microstructure were studied. The results show that as the voltage increases and the oxidation time...

Assessment of articular cartilage degeneration induced by degenerative diseases using quantitative ultrasound method is promising but challenging. Currently, in literature, proposed methods have limited diagnostic abilities, and some quantitative ultrasound cartilage evaluation results are inconsistent...

With the different treatment intensity of electric field, we analyze the influence of electric field on infrared spectra of superoxide dismutase by using infrared spectra method. The results show that, superoxide dismutase (SOD) is treated with different electric field intensity, the absorption peak...

The watermelon juice was pasteurized by the ultra-high temperature treatment, and stored at 4 øC for 2 weeks. The ultra-high temperature reduced the initial microbial counts of the watermelon juice. The aroma of the watermelon juice was reduced during the storage at 4 øC for 2 weeks. The typical volatiles...

In order to sucessfully prepare Na-MMT-Miglitol composite, the adsorption effect factors of the different concentrations, temperature , pH and time for miglitol were investigated respectively. By means of the adsorption capacity changes of Na-MMT, the maximum adsorption capacity of Na-MMT as the experimental...

The micro-morphology, wettability and chemical composition of the moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle (CA) meter and a Fourier transform infrared spectrometer (FT-IR). The micro-morphological models for hydrophobicity on the wing surface were...

In order to further study the Insecticidal Mechanism of Bt protein against Holotricia parallela. In the study, According to the results of midgut transcriptome sequencing and RACE-PCR, the full-length hpalp gene was cloned. Sequencing analysis showed that the open reading frame of hpalp (GenBank accession...