Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

DOI

10.2991/bbe-17.2017.2How to use a DOI?

Keywords

Recombinant follicle stimulating hormone (rFSH) , ELISA , HEK293 cells.

Abstract

Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study was to establish a method for screening cloned cell lines with high productivity from transfected HEK293 cells. Here, we adopt the antibiotics in combination with ELISA as a hyphenated technique to obtain production cell lines, and the results indicated that this a simple and efficient method in the cloned cell screening application.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jian-wen HU
AU  - Meng-meng LIU
AU  - Ji-zhong HAN
AU  - Bin ZENG
PY  - 2017/05
DA  - 2017/05
TI  - Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 5
EP  - 10
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.2
DO  - 10.2991/bbe-17.2017.2
ID  - HU2017/05
ER  -