Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals

DOI

10.2991/bbe-17.2017.27How to use a DOI?

Keywords

HKC cells, H2O2, Injury, Calcium oxalate, Nucleation, Growth.

Abstract

Purpose: The oxidative damage of hydrogen peroxide (H2O2) on human kidney proximal tubular epithelial cell (HKC) and the nucleation and growth of calcium oxalate (CaOx) crystals modulated by normal and injured cells were investigated. Methods: The method of CCK-8 was used to detect the proliferating inhibition effect of H2O2 on HKC. Cells were incubated with serum-free culture media containing CaOx supersaturated solutions. The cellular morphology and crystal growth were observed by invert microscope and scanning electron microscope (SEM). Results: H2O2 could damage HKC in time-dependent (0~60 min) and concentration-dependent manners (0~1.0 mmol/L). The amount of CaOx crystals induced by injured cells was more than that induced by control cells, and the size of the crystals induced by injured cells was also larger. An oxidative damage mode for H2O2 was established. Conclusions: HKC could be damaged obviously by both H2O2 and high concentration CaOx solution in time-dependent and concentration-dependent manners. Injured cells promote the nucleation and growth of CaOx crystals.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Da GUO
AU  - Jian-min WANG
AU  - Jian-ming OUYANG
PY  - 2017/05
DA  - 2017/05
TI  - Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 165
EP  - 170
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.27
DO  - 10.2991/bbe-17.2017.27
ID  - GUO2017/05
ER  -