Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves

DOI

10.2991/bbe-17.2017.37How to use a DOI?

Keywords

Hawthorn Leaves, Polysaccharide, Aqueous Two-Phase System, Ultrasound, Response Surfacemethodology.

Abstract

Aqueous two-phase system (ATPS) combined with ultrasonic technology was employed to extract polysaccharide from hawthorn leaves in this study. The partition behaviors in different ATPS were investigated first, polyethylene glycol (PEG) 11.5% and (NH4)2SO4 14% were selected as the optimum solvent for extracting polysaccharide. Using the optimum ATPS solvent, the ratio of material to solvent, The time, temperature and power of the ultrasonic treatment were investigated using a single factor experiment and Box-Behnken Design (BBD) of response surface methodology (RSM) to obtain the optimum conditions for extracting polysaccharide. The optimum conditions were the ratio of material to solvent 1:34, ultrasonic time (UT) 35 min, ultrasonic power (UP) 300 w and ultrasonic temperature (UTE) 65 . With the optimal conditions, the maximum yield of polysaccharide was 3.05%. The present study provided a foundation for further purification of polysaccharide and offered a valuable alternative for extracting polysaccharide from plants.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ri-li HAO
AU  - Juan-juan MA
AU  - Kun LI
AU  - Chuan-he ZHU
AU  - Xiao-cun ZHANG
PY  - 2017/05
DA  - 2017/05
TI  - Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 231
EP  - 237
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.37
DO  - 10.2991/bbe-17.2017.37
ID  - HAO2017/05
ER  -