Analysis of Malondialdehyde Levels in The Heart, Small Intestine, and Testes in White Rats Induced MSG with Treatment of Kesum Ethanol Extract (Polygonum minus Huds.)

DOI

10.2991/978-94-6463-457-0_19How to use a DOI?

Keywords

Kesum leaves; Malondialdehyde; Monosodium glutamate; Organs

Abstract

Monosodium glutamate (MSG) is a food additive used as a food flavoring. Excessive use of MSG can increase Reactive Oxygen Species which has an impact on rat organ damage, characterized by increased levels of malondialdehyde (MDA). Kesum leaves (Polygonum minus Huds.) contain antioxidants which can reduce MDA levels. Research Purpose to determine the effect of ethanol extract of kesum leaves on reducing MDA levels in the organs of white rats induced MSG. Method is experiment with post-test control group design. This study used 35 male Wistar rats divided into 5 groups, a Control (-) group without treatment, Control (+), P1, P2 and P3 were induced MSG 35 mg/Kg BW for 14 days and P1, P2 and P3 were given ethanol extract of kesum leaves for 7 days with 27.5; 55.0; 110.0 mg/200g rat body weight/day. MDA levels were measured using spectrophotometer. Data were analyzed using One Way ANOVA and Tukey’s Post Hoc test. Results MDA levels in the heart, small intestine and testes of mice induced MSG were 6.6020; 15.966; 5.58867 mg/kg. MDA levels in the three organs of mice induced by MSG, then given ethanol extract of kesum leaves (P1, P2, and P3) decreased. The level of kesum leaf ethanol extract which can reduce MDA levels is a maximum of 60%; 92%; 83% is a concentration of 110.0 mg/200gbw rat. Post Hoc Tukey test results show that K(+) is different from P1 (p=0.007), P2 (p=0.000), and P3 (p=0.000). There was an effect of variations in the concentration of kesum leaf ethanol extract on reducing MDA levels. The final conclusion is ethanol extract of kesum leaves (Polygonum minus Huds.) can reduce MDA levels in the organs of white rats were induced MSG.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ana Hidayati Mukaromah
AU  - Fadli Sukardiansyah
AU  - Nadia Mulyana Kum
AU  - Muhammad Renaldi
AU  - Chatarina Hesty Nugraheni
PY  - 2024
DA  - 2024/07/22
TI  - Analysis of Malondialdehyde Levels in The Heart, Small Intestine, and Testes in White Rats Induced MSG with Treatment of Kesum Ethanol Extract (Polygonum minus Huds.)
BT  - Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)
PB  - Atlantis Press
SP  - 159
EP  - 168
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-457-0_19
DO  - 10.2991/978-94-6463-457-0_19
ID  - Mukaromah2024
ER  -