Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)

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Pfmdr1 Gene Polymorphism in Plasmodium falciparum Towards First-Line Treatment Resistance of Malaria Patients of Marthen Indey Jayapura Hospital

Authors
Indah Genesya Callin Makienggung1, *, Aditya Rahman Ernanto1, Aprilia Indra Kartika1
1Universitas Muhammadiyah Semarang, Semarang, Central Java, 50273, Indonesia
*Corresponding author.
Corresponding Author
Indah Genesya Callin Makienggung
Available Online 22 July 2024.
DOI
10.2991/978-94-6463-457-0_8How to use a DOI?
Keywords
Antimalaria; Resistance; Plasmodium falciparum; Polymorphism; Pfmdr1 Gene
Abstract

Malaria is caused by a parasitic infection of the genus Plasmodium. Plasmodium falciparum is the cause of almost all deaths caused by malaria. Malaria problem has been exacerbated in recent years by the development and rapid spread of P. falciparum resistance to antimalarial drugs. Resistance to antimalarial drugs is caused by genetic factors from P. falciparum, one of which is due to polymorphisms in the Pfmdr1 gene. Pfmdr1 gene is a potential marker of resistance to several antimalarial drugs. The aim of this study was to detect the Pfmdr1 gene polymorphism in P. falciparum for resistance to first-line treatment in malaria sufferers. This type of research was an observational research. The method used is PCR and direct sequencing. Samples used were 9 resistant samples and 1 negative control sample in the form of whole blood. The results of the study showed that samples 1 to 9 formed DNA bands with a size of 590 bp targeting the Pfmdr1 gene. The sequencing results analysed at codon 86 showed no changes in nucleotide bases or amino acids, so no polymorphism occurred in the sample. This suggests that the polymorphism in the Pfmdr1 codon 86 gene was not the cause of resistance to first-line antimalarial treatment in falciparum malaria patients Dihydroartemisinin-Piperaquine (DHP) + Primaquine.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)
Series
Advances in Biological Sciences Research
Publication Date
22 July 2024
ISBN
10.2991/978-94-6463-457-0_8
ISSN
2468-5747
DOI
10.2991/978-94-6463-457-0_8How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Indah Genesya Callin Makienggung
AU  - Aditya Rahman Ernanto
AU  - Aprilia Indra Kartika
PY  - 2024
DA  - 2024/07/22
TI  - Pfmdr1 Gene Polymorphism in Plasmodium falciparum Towards First-Line Treatment Resistance of Malaria Patients of Marthen Indey Jayapura Hospital
BT  - Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)
PB  - Atlantis Press
SP  - 65
EP  - 74
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-457-0_8
DO  - 10.2991/978-94-6463-457-0_8
ID  - Makienggung2024
ER  -