Curcuma zanthorrhiza Extracts Induce G2/M Cell Cycle Arrest and Apoptosis in 4T1 and MCF-7 Human Breast Cancer Cells
- DOI
- 10.2991/ahsr.k.200215.079How to use a DOI?
- Keywords
- Curcuma zanthorrhiza, cell cycle, apoptosis
- Abstract
Curcuma zanthorrhiza Roxb., known as Javanese turmeric or temulawak in Indonesia, has been reported to have several biological activities, including anticarcinogenic effects. In this study, we aimed to evaluate the effects of temulawak rhizome extract on the cell viability, cell cycle distribution, apoptosis induction, and antimigratory effect of human breast cancer cell line. The rhizome powdre was macerated using ethanol 96% to obtain dry extract. The cell lines (MCF-7 and 4T1) were subjected to increasing doses of temulawak extract and cell viability was quantified by MTT assay. Cell cycle profile and apoptosis induction were done using flow cytometry assay. In vitro wound healing assay was performed on 4T1 cells to evaluate antimigratory effect. Temulawak extract inhibited the growth of MCF-7 and 4T1 cells with the IC50 value 46 and 37 μg/ml respectively. Temulawak extracts induced cell cycle arrest in G2/M phase and apoptosis in MCF-7 and 4T1 cells. The extract was not show anti-migration effect in wound healing assay. This study confirms the potential cytotoxic effects of temulawak in breast cancer cell line and could be developed as a potential chemopreventive agent.
- Copyright
- © 2020, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Sari Haryanti AU - Galuh Ratnawati AU - Nuning Rahmawati PY - 2020 DA - 2020/02/22 TI - Curcuma zanthorrhiza Extracts Induce G2/M Cell Cycle Arrest and Apoptosis in 4T1 and MCF-7 Human Breast Cancer Cells BT - Proceedings of the 4th International Symposium on Health Research (ISHR 2019) PB - Atlantis Press SP - 415 EP - 419 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.200215.079 DO - 10.2991/ahsr.k.200215.079 ID - Haryanti2020 ER -