Activity Test and Inhibitory Mechanism of Essential Oil of Lime Leaf (Citrus Aurantifolia, Swingle) Against Some of Bacterial Pathogens

DOI

10.2991/icosat-17.2018.11How to use a DOI?

Keywords

Essential oil of lime leaf, Antibacterial, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, Bacillus subtilis.

Abstract

Lime fruit (Citrus aurantifolia Swingle) is traditionally known to be useful for the treatment of various infectious diseases like fever, cough, toothache, sore throat, diphtheria, body odor, ringworm etc as folk medicine. Objectives: This study aims to determine the activity and mechanism of inhibition of the growth of some bacterial pathogens by the essential oil of lime leaves (Citrus aurantifolia, swingle). Materials and Methods: The lime leaves was obtained from Balitro Bagor, distilled by steam distillation process. The essential oil of lime leaf that obtained was analyzed by Gas Chromatography-Mass Spectroscopy. The activity test of anti-bacterial of essential oil of lime leaves was carried out by using the method mikrodilution against the bacterium Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus and Bacillus subtilis. For detect the mechanism of cell damage process of bacteria was observed leakage of proteins and nucleic acids by using Ultra Violet-Visible spectrophotometer and the leakage of cations K+ and Ca2+ was observed by using Atomic Absosrtion Spectrum. While bacterial cell morphology change was observed by using Scanning Electron Microscope (SEM). Results and Conclusion: From the research was obtained, Lime leaf contains about 0.4% (v/w) essential oils with the main components are geranial (10.39%), limonene (10.2%), neral (8.94%), kariofilena (5.72%) and citronellal (5.41%). The essential oil of lime leaf has the highest antibacterial activity on the bacteria tested was on Bacillus subtilis, with a minimum inhibitory concentration (MIC) of 0.125% (v/v). Test results nucleic acid and protein leakage and cations leakage of K+ and Ca2+ greatly increased from 1 MIC to 2 MIC concentration of the tested bacteria compared with the normal controls. While the observations that was detected by using Scanning Electron Microscope (SEM) showed worse cell damage by giving the higher doses to bacteria.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Muhammad Yanis Musdja
AU  - Arys Medta Pariwidjayanti
AU  - Andria Agusta
PY  - 2017/08
DA  - 2017/08
TI  - Activity Test and Inhibitory Mechanism of Essential Oil of Lime Leaf (Citrus Aurantifolia, Swingle) Against Some of Bacterial Pathogens
BT  - Proceedings of the International Conference on Science and Technology (ICOSAT 2017)
PB  - Atlantis Press
SP  - 42
EP  - 50
SN  - 1951-6851
UR  - https://doi.org/10.2991/icosat-17.2018.11
DO  - 10.2991/icosat-17.2018.11
ID  - Musdja2017/08
ER  -