Expression Of Human Neutrophil Gelatinase-Associated Lipocalin (Ngal) On Serum And Urine Sample Of Pre Dialysis Kidney Failure Patients

DOI

10.2991/icoh-17.2018.10How to use a DOI?

Keywords

Human Neutrophil gelatinase-associated lipocalin (NGAL), Kidney Failure, Predialisa, SDS PAGE, Westernblot

Abstract

Biomarkers for the early prediction of human acute kidney injury (AKI) are clinically important. Recently, neutrophil gelatinase-associated lipocalin (NGAL) has found to be a sensitive biomarker for the prediction of human AKI at a very early stage and the development of AKI after surgery. NGAL is an accurate biosensor to determine kidney function, previous studies have mentioned that NGAL biomarker has been detected in patients with renal failure post cardiac surgery, kidney transplants, and septicemia shock. Therefore the objective of this study is to evaluate the expression of NGAL in serum and urine sample of patients with predialysis renal failure.We collected 10 samples of patients predialysis in Saiful Anwar Hospital which were indicated kidney failure. Then NGAL protein expression was evaluated by SDS PAGE and verified by western blot method. Urea and creatinine serum were analyzed by spectrophotometer method. The data obtained were analyzed descriptively. The results showed that in 10 patients with renal failure predialysis have shown an increase of serum BUN and creatinine above normal. Serum and urine samples of patients were examined by polyacrylamide gel electrophoresis method by a thick protein bands at 25 kDa. While the western blot of NGAL showed positive results expressed by NGAL protein in the samples. In conclusion, the NGAL protein was detected in predialysis patients.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Retty Ratnawati
AU  - Tinny Endang Hernowati
AU  - Nurina Titisari
AU  - Ahmad Fauzi
PY  - 2017/07
DA  - 2017/07
TI  - Expression Of Human Neutrophil Gelatinase-Associated Lipocalin (Ngal) On Serum And Urine Sample Of Pre Dialysis Kidney Failure Patients
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 42
EP  - 45
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.10
DO  - 10.2991/icoh-17.2018.10
ID  - Ratnawati2017/07
ER  -