Optimization of Multiplex PCR for Detection of Pork-Contaminated Beef-Based Food Product

DOI

10.2991/978-94-6463-166-1_68How to use a DOI?

Keywords

Multiplex PCR; Food Authentication; ND5; Sus scrofa; Bos taurus

Abstract

The development of globalization affects Indonesia, especially science and technology in the field of food biotechnology. Molecular analysis method, namely Polymerase Chain Reaction (PCR) can be used to detect the food authentication. One of the modifications developed from the conventional PCR method is multiplex PCR which can amplify more than one target gene simultaneously in one PCR reaction. In order to develop an efficient and effective in-house method in detecting food product falsification, such as pork-contaminated beef-based food product, this research aims to obtain the optimum multiplex PCR conditions to amplify the ND5 gene of pork and beef simultaneously by optimizing variations in the primer concentration of pork ND5 primer pairs (Sus-F and Sus-R), beef ND5 primers (Bos-F and Bos-R) and the PCR program. The results of this study showed that the use of 0.5 μ M pairs of Sus primers and 0.3 μ M pairs of Bos primers in the PCR reaction and the use of the multiplex PCR program at annealing temperatures of 58.7 ℃ and 48 ℃ were able to detect mixing of pork DNA and beef simultaneously, according to the size of amplicon for pork was 467 bp and for beef was 206 bp.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Moralita Chatri
AU  - Afifatul Achyar
AU  - Cahay Kamila Putri
PY  - 2023
DA  - 2023/05/22
TI  - Optimization of Multiplex PCR for Detection of Pork-Contaminated Beef-Based Food Product
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 567
EP  - 573
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_68
DO  - 10.2991/978-94-6463-166-1_68
ID  - Chatri2023
ER  -