The Immunostimulant Activities of the Gambir (Uncaria gambir Roxb) on Raw 264.7 Cell
- DOI
- 10.2991/ahsr.k.211105.041How to use a DOI?
- Keywords
- Uncaria gambir Roxb; RAW 264.7 cell; cell viability; immunostimulant
- Abstract
Gambir (Uncaria gambir Roxb) is a plant that is known to have various activities, one of which is immunostimulant. This study aims to determine whether gambir from Payakumbuh affects the viability and immunostimulant activity on RAW 264.7 cells induced by lipopolysaccharide (LPS). This study was conducted in vitro with a concentration 1, 10, 100μg/mL on the viability and immunostimulatory activity of RAW 264.7 cells. Cell viability testing using the microtetrazolium (MTT) method aims to see whether the gambir sample used is safe and not toxic on RAW 264.7 cells by looking at the cell viability value >90%. Gambir 1,10, 100μg/mL was safe and non-toxic to RAW 264.7 cells with a viability value >90%. Thus, the concentration of gambir used can be further tested, namely the immunostimulant activity of gambir on RAW 264.7 cells induced by LPS looking at the levels of IL-6 (proinflammatory cytokines) compared with LPS as a control using the sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It was found that gambir 1,10, 100μg/mL could significantly increase the levels of IL-6 (p<0,05) which was tested with one-way ANOVA and continued with Duncan’s post hoc test. Based on these results, it can be concluded that gambir from Payakumbuh (Uncaria gambir Roxb) is safe and non-toxic and has immunostimulant activity by increasing the levels of IL-6 which were tested on RAW 264.7 cells induced by LPS.
- Copyright
- © 2021 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Elsya Gustia AU - Yufri Aldi AU - Dira Hefni AU - Sefrianita Kamal AU - Dachriyanus AU - Fatma Sri Wahyuni PY - 2021 DA - 2021/11/17 TI - The Immunostimulant Activities of the Gambir (Uncaria gambir Roxb) on Raw 264.7 Cell BT - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021) PB - Atlantis Press SP - 282 EP - 288 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.211105.041 DO - 10.2991/ahsr.k.211105.041 ID - Gustia2021 ER -