Antibacterial Activity of Ethyl Acetate Extracts from Mangrove Plants Rhizophora apiculata and Sonneratia alba — Associated Fungi
- DOI
- 10.2991/ahsr.k.211105.049How to use a DOI?
- Keywords
- Mycobacterium tuberculosis; Aspergillus flavus; Aspergillus aculeatus; Penicillium rubens
- Abstract
This study aims to explore the source of antibacterial compounds from mangrove endophytic fungi. Nine fungi were isolated from the leaves, barks, and roots of mangrove Rhizophora apiculata and Sonneratia alba from West Sumatera, Indonesia. The fungal isolate was cultivated in rice for 4-6 weeks, and subsequently, its secondary metabolites were extracted using ethyl acetate. The ethyl acetate extracts were tested against pathogenic bacteria; Bacillus cereus, Multi-Drug Resistant Pseudomonas aeuruginosa, Staphylococcus aureus, and Escherichia coli, and Mycobacterium tuberculosis. Seven extracts showed antibacterial activity at 5% (b/v %) concentration in the diffusion method. The highest inhibition was exhibited by the extract of RAA1 with the range of inhibition zone of 21.87 ± 0.81 mm – 26.12 ± 0.21 mm. Six fungal extracts (RAA2, RAA3, RAA4, RAK2, SaDa1, SaDa2) showed sensitivity to M. tuberculosis at concentrations of 3% and 5%. The potential fungi were identified molecularly by DNA amplification and sequencing of the fungal ITS region. The results of identification showed RAA1 as Aspergillus flavus, RAA4 as Aspergillus aculeatus, and SaDa1 as Penicillium rubens.
- Copyright
- © 2021 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - R Dyana Sartika AU - Annisa Rahma AU - Salsabillah Amelano AU - Dian Handayani PY - 2021 DA - 2021/11/17 TI - Antibacterial Activity of Ethyl Acetate Extracts from Mangrove Plants Rhizophora apiculata and Sonneratia alba — Associated Fungi BT - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021) PB - Atlantis Press SP - 336 EP - 342 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.211105.049 DO - 10.2991/ahsr.k.211105.049 ID - DyanaSartika2021 ER -