Optimization, Production, and Partial Purification of Protease from Halotolerant Actinomycetes Nocardiopsis dassonvillei

DOI

10.2991/978-94-6463-648-2_7How to use a DOI?

Keywords

Halotolerant actinomycetes; Nocardiopsis dassonvillei; 16S rRNA sequencing; BLAST analysis; SDS electrophoresis

Abstract

The exploration of halotolerant actinomycetes for industrial enzyme production, particularly proteases, presents significant potential due to their resilience in extreme environments. The primary aim of this study was to screen for protease-producing halotolerant actinomycetes, optimize the conditions for enzyme production, and partially purify the protease for potential applications. To achieve this, halotolerant actinomycetes were isolated from Kelambakkam salt pan about 35 km from Chennai and subjected to primary screening on various media to detect protease activity. Among 32 actinomycete isolates, only MML2025 demonstrated protease production, exhibiting the ability to hydrolyze gelatin substrate. The colony morphology of this isolate was examined to identify distinguishing features. Several factors, including NaCl concentration, pH, and temperature, were optimized to enhance protease production. The strain MML2025, which showed the highest protease production, was identified as Nocardiopsis dassonvillei through 16S rRNA sequencing and BLAST analysis. Mass production of protease was subsequently carried out under optimized conditions, followed by partial purification using ammonium sulfate precipitation and dialysis to concentrate the enzyme and remove impurities. The crude partially purified protease was analyzed using SDS electrophoresis, revealing apparent protein molecular weights of 43 kDa, 25 kDa, and 18 kDa. Nocardiopsis dassonvillei showed substantial protease production and salt tolerance, underscoring its promise as a source for industrial enzymes. This study is the first to confirm protease production by this actinomycete, making it a significant finding in the field.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - S. Sugitha
AU  - M. Suganthi
AU  - K. Ashok Kumar
AU  - M. Jayanthi
AU  - C. Ramprasath
AU  - G. Abirami
PY  - 2025
DA  - 2025/02/07
TI  - Optimization, Production, and Partial Purification of Protease from Halotolerant Actinomycetes Nocardiopsis dassonvillei
BT  - Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
PB  - Atlantis Press
SP  - 69
EP  - 87
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-648-2_7
DO  - 10.2991/978-94-6463-648-2_7
ID  - Sugitha2025
ER  -