Cytotoxic Activity and Antiproliferation of Soursop Seed (Annona muricata L.) Protein on MCF-7 Cells
- DOI
- 10.2991/978-94-6463-050-3_30How to use a DOI?
- Keywords
- Soursop Seed; RIPs; Cytotoxic; Antiproliferation
- Abstract
One of the new anticancer treatments that can be developed is a protein similar to RIPs. RIP is an enzyme found in various parts of plants that is toxic and can inhibit protein synthesis. Soursop seeds (Annona muricata L.) may be developed into anticancer. The plant of the same genus, Annona Squamosa L., has been proven to have a protein content similar to that of RIPs because it can supercoil DNA into circular nicks at low levels. Another study reported that soursop seed protein had moderate cytotoxic activity against 4T1 cells but did not show any antiproliferative activity. This study was conducted to determine the cytotoxic and antiproliferative activity of soursop seed (Annona muricata L.) protein against MCF-7 cells. Cytotoxic and antiproliferative activity tests were carried out using the MTT method on the protein fraction of soursop seeds. Cytotoxicity test was performed within 24 h. The antiproliferative test was carried out for 24, 48, and 72 h with the test parameter, doubling time. The results of the cytotoxicity test obtained that the protein fraction had weak cytotoxic activity with an IC50 value of 429,90 µg /mL. The antiproliferative results implied that there was no growth inhibition as indicated by the concentration of the protein fractions of 7,5 µg/mL and 3,75 µg/mL, which were unable to prolong the time twice compared to control cells. The results of this study showed that the protein fractions of soursop seeds did not have antiproliferative activity against MCF-7 cells.
- Copyright
- © 2023 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Itsna Sofia Rahma AU - Peni Indrayudha AU - Maryati AU - Azis Saifudin AU - Cita Hanif Muflihah PY - 2022 DA - 2022/12/14 TI - Cytotoxic Activity and Antiproliferation of Soursop Seed (Annona muricata L.) Protein on MCF-7 Cells BT - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022) PB - Atlantis Press SP - 349 EP - 360 SN - 2590-3195 UR - https://doi.org/10.2991/978-94-6463-050-3_30 DO - 10.2991/978-94-6463-050-3_30 ID - Rahma2022 ER -