Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)

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Cytoxicity of Melinjo (Gnetum Gnemon L.) Seed Protein on MCF-7 and Vero Cells and Its Antiproliferation Activity

Authors
Sonia Tithaning Sukma1, Peni Indrayudha1, *, Maryati1, Azis Saifudin1, Cita Hanif Muflihah1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani Tromol Pos I, Pabelan, 57162, Kartasura, Sukoharjo, Indonesia
*Corresponding author. Email: peni.indrayudha@ums.ac.id
Corresponding Author
Peni Indrayudha
Available Online 14 December 2022.
DOI
10.2991/978-94-6463-050-3_29How to use a DOI?
Keywords
Melinjo; MCF-7; Vero; Cytotoxic; Antiproliferative; Selectivity
Abstract

Melinjo seeds are one of the plants that have the potential to be developed as anticancer drugs. This study aimed to determine the cytotoxic activity of melinjo seed protein against MCF-7 and vero cells, and to determine the antiproliferative activity of melinjo seed protein against MCF-7 cells. The method used for the isolation of melinjo seed protein was SDS-PAGE. Cytotoxic and antiproliferative activities were carried out using the MTT assay method and the absorbance was read on an ELISA reader. The results showed that melinjo seed protein had cytotoxic activity against MCF-7 cells with IC50 of 125.89 µg/mL. The IC50 results of melinjo seed protein against vero were 3.37 µg/mL. Melinjo seed protein was not selective against vero cells where the SI values were 0.03. In the antiproliferative activity test of melinjo seed protein, the doubling time at levels of 1.875 µg/mL was 86.55 h, while the control cell doubling time was 112.32 h. These results indicated that melinjo seed protein was not able to inhibit the proliferation of MCF-7 cells.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
Series
Atlantis Highlights in Chemistry and Pharmaceutical Sciences
Publication Date
14 December 2022
ISBN
978-94-6463-050-3
ISSN
2590-3195
DOI
10.2991/978-94-6463-050-3_29How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Sonia Tithaning Sukma
AU  - Peni Indrayudha
AU  - Maryati
AU  - Azis Saifudin
AU  - Cita Hanif Muflihah
PY  - 2022
DA  - 2022/12/14
TI  - Cytoxicity of Melinjo (Gnetum Gnemon L.) Seed Protein on MCF-7 and Vero Cells and Its Antiproliferation Activity
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 339
EP  - 348
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_29
DO  - 10.2991/978-94-6463-050-3_29
ID  - Sukma2022
ER  -