Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)

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Cytotoxic Activity and Antiproliferation of Soursop Seed Protein (Annona muricata) Against 4T1 Cells

Authors
Monarita Puspita Dewi1, Peni Indrayudha1, *, Maryati1, Azis Saifudin1, Cita Hanif Muflihah1
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani Tromol Pos I, Pabelan, Kartasura, Sukoharjo, 57162, Indonesia
*Corresponding author. Email: peni.indrayudha@ums.ac.id
Corresponding Author
Peni Indrayudha
Available Online 14 December 2022.
DOI
10.2991/978-94-6463-050-3_31How to use a DOI?
Keywords
Annona muricata; MTT assay; 4T1 cells; antiproliferative assay; cytotoxic assay
Abstract

Current cancer treatments still have a lot of negative impacts, so it is necessary to find alternative treatments that come from nature or plants that have anti-cancer activity. Plants that have the potential to be anti-cancer agents are plants that contain Ribosome-Inactivating Proteins (RIPs). Soursop plants are candidates for this experiment because the same genus as soursop, sugar apple (Annona squamosa L.), has shown RIPs activity. This study aimed to determine the antiproliferative activity of soursop seed protein (Annona muricata) against 4T1 cells. Protein isolation used the ion-exchange chromatography method. Cytotoxicity and antiproliferation tests used the MTT assay method, after obtaining the IC50 value, an antiproliferative test was conducted with concentrations below IC50 with incubation times of 24, 48, and 72 h. The cytotoxicity test results of the soursop protein fraction were 73.96 ug/mL ± 25.10, and doxorubicin had an IC50 value of 5.067 ug/mL. The results of the control antiproliferative test were 100.33 h, while the test group was 77.44 h. The results of the test indicate that the soursop seed fraction was not able to inhibit the proliferation of 4T1 cells.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
Series
Atlantis Highlights in Chemistry and Pharmaceutical Sciences
Publication Date
14 December 2022
ISBN
978-94-6463-050-3
ISSN
2590-3195
DOI
10.2991/978-94-6463-050-3_31How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Monarita Puspita Dewi
AU  - Peni Indrayudha
AU  - Maryati
AU  - Azis Saifudin
AU  - Cita Hanif Muflihah
PY  - 2022
DA  - 2022/12/14
TI  - Cytotoxic Activity and Antiproliferation of Soursop Seed Protein (Annona muricata) Against 4T1 Cells
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 361
EP  - 369
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_31
DO  - 10.2991/978-94-6463-050-3_31
ID  - Dewi2022
ER  -