Analysis of DNA Expression of Mycobacterium Tuberculosis in Formalin-Fixed Paraffin-Embedded (FFPE) Granulomatous Mastitis

DOI

10.2991/978-2-38476-110-4_33How to use a DOI?

Keywords

Granulomatous Mastitis; Tuberculous Mastitis; Mycobacterium tuberculosis; FFPE; PCR; MTB

Abstract

Granulomatous Mastitis (GM) is a chronic inflammatory breast disease characterized by sterile noncaseating granulomatous inflammation with etiopathogenesis that has not yet been fully evaluated. Tuberculous mastitis is a chronic infectious disease caused by bacillus Mycobacterium tuberculosis (MTB) and is also one of the differential diagnoses of GM. In developing countries such as Indonesia, the most common cause of granuloma is Mycobacterium tuberculosis infection, besides leprosy, foreign body granuloma, fungal infection, parasitic infection, and actinomycosis. The gold standard for diagnosing tuberculous mastitis is the detection of MTB in a specimen. This study aimed to analyze DNA expression of Mycobacterium tuberculosis using PCR technique on formalin-fixed and paraffin-embedded (FFPE) samples of granulomatous mastitis. A total of 45 FFPE samples were collected and subjected to histopathological examination (using Hematoxylin Eosin and Ziehl Neelsen staining) and DNA extraction (Invitrogen Purelink Genomic DNA Mini Kit). A conventional PCR was conducted using the IS6110 gene as target to detect MTB DNA. All GM patients in this study were women with an average age of 30.8 years. All FFPE samples of GM tested negative in the PCR, and no 123 bp band was observed. This suggests that Granulomatous mastitis can be caused by MTB or Mott, and conventional PCR is not sensitive to detect Mycobacterium tuberculosis in FFPE samples of GM.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Fairuz
AU  - H. Dewi
AU  - E. A. Utami
AU  - Lipinwati
PY  - 2023
DA  - 2023/11/07
TI  - Analysis of DNA Expression of Mycobacterium Tuberculosis in Formalin-Fixed Paraffin-Embedded (FFPE) Granulomatous Mastitis
BT  - Proceedings of the 4th Green Development International Conference (GDIC 2022)
PB  - Atlantis Press
SP  - 306
EP  - 315
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-110-4_33
DO  - 10.2991/978-2-38476-110-4_33
ID  - 2023
ER  -