The Screening of Growing Media for Acclimatization of Banana Plantlets

DOI

10.2991/978-2-38476-110-4_34How to use a DOI?

Keywords

Plant propagation; In vitro culture; Organic fertilizer; Musa spp

Abstract

Media for acclimatization is one of the important factors for the success of plant propagation through tissue culture techniques, because the regenerated plantlets do not yet have a well-developed root system. This situation causes plantlets to be very sensitive to environmental stresses, both abiotic and biotic. Therefore, plantlet acclimatization requires special handling, and modifications to environmental conditions are needed, especially in relation to growing media. This study aimed to obtain the best media composition and dose of arbuscular mycorrhizal fungi (AMF) to support the growth of banana plantlets during the acclimatization stage. This investigation was conducted at the Plant Biotechnology Laboratory Faculty of Agriculture University of Jambi for approximately 4 months. Tissue culture generated plantlets of banana cv. Barangan were acclimatisized on media consisted of s-oil and trichocompost (1:1, 1:2 and 2:1) and AMF (0, 10, and 20 g per plantlet). The trial was arranged in a factorial randomized block design with 3 replications, making the total of 27 experimental units. Each unit consisted of 10 polybags containing one individual plant. Data were recorded on the percentage of live seedlings (survival rate), increase in plant height, increase in number of leaves, increase in stem diameter, length, and width of fully developed leaves. Statistical analysis using Analysis of Variance, followed by the Least Significant Difference test at 5% confidence interval was employed to see the effect of the media compositions and AMF on plantlet growth. Results showed that all transplanted plantlets suceeded acclimatization process (100% survival rate). Statistical analysis revealed that either the combination of media compositions and dosages of AMF or AMF alone did not show any significant effect on all variables observed. The media composition, however, was found to have significant effect on plant height, leaf number, and leaf length, but not on stem diameter and leaf width. This investigation confirmed benefit of the use of trichocompost in acclimatization media, in which the composition of 2:1 of soil and trichocompost gave the best the growth of banana plantlets during acclimatization.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Zulkarnain Zulkarnain
AU  - Eliyanti Eliyanti
AU  - Elis Kartika
AU  - Budiyati Ichwan
PY  - 2023
DA  - 2023/11/07
TI  - The Screening of Growing Media for Acclimatization of Banana Plantlets
BT  - Proceedings of the 4th Green Development International Conference (GDIC 2022)
PB  - Atlantis Press
SP  - 316
EP  - 324
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-110-4_34
DO  - 10.2991/978-2-38476-110-4_34
ID  - Zulkarnain2023
ER  -