T(-786)C Polymorphism of NOS3 Gene in the Yakut Population

DOI

10.2991/ahsr.k.220103.042How to use a DOI?

Keywords

T(-786)C Polymorphism; Endothelial NOS; NOS3 gene; Yakut population

Abstract

Endothelial NOS (nitric oxide synthase 3) is an enzyme that synthesizes nitric oxide, which in turn plays a crucial role in regulating vascular tone, cell proliferation, leukocyte adhesion, and platelet aggregation. This enzyme in humans is encoded by NOS3 gene. The aim of our research was to study T (-786) C polymorphism of NOS3 gene in healthy individuals of the Yakut population. The experimental part of the research was carried out in “Molecular Medicine and Human Genetics” of the NEFU Medical Institute. In total, 270 DNA samples of healthy Yakuts were examined (71 men: women 199, average age 49.18 ± 14.36 years). The single nucleotide polymorphism was determined by real-time PCR with fusion of fluorescent labeled hybridization products followed by analysis of melting curves. As a result of genotyping T (-786) C polymorphism of NOS3 gene, the predominance of T allele (95.37 %) was established among the Yakut population. Among all examined patients, carriers of the homozygous TT genotype were dominated – 246 people (91.11 %). The heterozygous TC genotype was 8.52 % (23), whereas the homozygous C allele genotype occurred only in 0.37 % of cases (1). When compared with populations of the world, according to the “1000 genomes,” C allele among the Yakuts was less common than among the population of North America, South America, Europe, India, Oceania, Africa and Asia. Thus, the Yakut population is characterized by a unique distribution of genotypes of T (-786) C polymorphism of NOS3 gene among populations of the world.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Julia Solovieva
AU  - Natalia Borisova
PY  - 2022
DA  - 2022/01/17
TI  - T(-786)C Polymorphism of NOS3 Gene in the Yakut Population
BT  - Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021)
PB  - Atlantis Press
SP  - 209
EP  - 213
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.220103.042
DO  - 10.2991/ahsr.k.220103.042
ID  - Solovieva2022
ER  -