Proceedings of the 2023 Brawijaya International Conference (BIC 2023)

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Genetic Diversity and Molecular Analysis of Hybrid Melon (Cucumis melo L.) genotype Based on Simple Sequence Repeats (SSR) Markers

Authors
Afifuddin Latif Adiredjo1, *, Radin Bayu Putra Ajidin2
1Faculty of Agriculture, Universitas Brawijaya, Malang, Indonesia, 65145
2Faculty of Agriculture, Universitas Brawijaya, Malang, Indonesia, 65145
*Corresponding author. Email: al.adiredjo@ub.ac.id
Corresponding Author
Afifuddin Latif Adiredjo
Available Online 29 October 2024.
DOI
10.2991/978-94-6463-525-6_24How to use a DOI?
Keywords
SSR; DNA; Genetic Diversity
Abstract

This study was carried out to identify the potential hybrid melon genotypes with analysis of genetic diversity using the Simple Sequence Repeats (SSR) Marker. Information from the results of genetic diversity analysis can be used to find information on the appearance of several potential hybrid varieties and to support data or confirmation from the results of genetic diversity based on morphological tests. The research was conducted at the Laboratory of Biotechnology, Faculty of Agriculture, University of Brawijaya, from February to May 2023. Research activities included taking leaf samples from 5 melon genotypes, DNA isolation, DNA quality testing, and amplification with SSR markers. Five SSR markers were polymorphic in all genotypes, revealing a total of 29 alleles. The five hybrid genotypes tested produced different DNA band lengths that indicated genetic variation or diversity. Based on the results of principal component analysis with R studio, the distribution of the genotypes of the five melon hybrids spread over four different plots. Genotypes that have a wide distribution or are in different plots indicate a distant kinship relationship and a high level of genetic diversity. The results of principal component analysis also show that there are two main components formed with eigenvalues of 1.88 and 1.01 respectively with a cumulative diversity value of 91.31%.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2023 Brawijaya International Conference (BIC 2023)
Series
Advances in Economics, Business and Management Research
Publication Date
29 October 2024
ISBN
978-94-6463-525-6
ISSN
2352-5428
DOI
10.2991/978-94-6463-525-6_24How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Afifuddin Latif Adiredjo
AU  - Radin Bayu Putra Ajidin
PY  - 2024
DA  - 2024/10/29
TI  - Genetic Diversity and Molecular Analysis of Hybrid Melon (Cucumis melo L.) genotype Based on Simple Sequence Repeats (SSR) Markers
BT  - Proceedings of the 2023 Brawijaya International Conference (BIC 2023)
PB  - Atlantis Press
SP  - 211
EP  - 219
SN  - 2352-5428
UR  - https://doi.org/10.2991/978-94-6463-525-6_24
DO  - 10.2991/978-94-6463-525-6_24
ID  - Adiredjo2024
ER  -