Effect of Different Extraction Methods and Types of Solvent on Bintaro (Cerbera manghas) Leaf Extract

DOI

10.2991/978-94-6463-525-6_25How to use a DOI?

Keywords

Alkaloids; Bintaro; Cerbera manghas; maseration; Ultrasonic Assisted Extraction

Abstract

Bintaro (Cerbera manghas) is a mangrove plant family that can be found growing on the beach in barren land. The Bintaro tree has excellent exploration potential because it contains many alkaloids, namely cerberin. Alkaloids are secondary metabolites that are widely used in the pharmaceutical, medical, and industrial fields. Cerberin is a natural pesticide because it has cytotoxic, antibacterial, and depressant properties. Alkaloids can be obtained through the extraction of non-thermal plant tissues such as maceration because they are not heat resistant using polar solvents such as distilled water and ethanol. In this study, two types of extraction were used, namely maceration and Ultrasonic Assisted Extraction (UAE) using three types of solvents, which are acetone, ethanol, and methanol. The purpose of this study is to determine the best combination of solvent use and extraction method in bintaro leaf extraction in terms of yield, refractive index value, and total alkaloid content. This research was conducted with a nested experimental design, namely the use of nested solvents in the use of extraction methods. Each treatment was repeated 3 times so that there were 18 experimental units. Statistical tests were carried out using normality, homogeneity, and ANOVA tests, and continued with the selection of the best treatment using the Zeleny Multiple Attribute method. The calculation of total alkaloid content was carried out using UV-VIS spectrophotometry so that a standard curve using caffeine compounds was needed. By making a caffeine standard curve, a line equation was created that is y = 0.0045 x + 0.0787 with a value of r2 = 0,9547. The results of this study indicate that in the yield parameters, refractive index, and alkaloid content, the best treatment combination is the use of 70% methanol solvent with UAE method with an average of 2.374 ± 0.05 for the yield, 1.480 ± 0.03 for the refractive index value, and 3.827 ± 0.09 for the alkaloid content parameter. After calculating the best treatment, the best treatment was obtained using 70% methanol solvent with UAE extraction.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Hendrix Y. Setyawan
AU  - Beauty S. D. Dewanti
AU  - Jemima A. P. Ariandini
AU  - Devy Ulandari
AU  - Shafira A. Sundari
PY  - 2024
DA  - 2024/10/29
TI  - Effect of Different Extraction Methods and Types of Solvent on Bintaro (Cerbera manghas) Leaf Extract
BT  - Proceedings of the 2023 Brawijaya International Conference (BIC 2023)
PB  - Atlantis Press
SP  - 220
EP  - 229
SN  - 2352-5428
UR  - https://doi.org/10.2991/978-94-6463-525-6_25
DO  - 10.2991/978-94-6463-525-6_25
ID  - Setyawan2024
ER  -