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Volume 1, Issue 5, October 2020, Pages 369 - 376

Simultaneous Detection of Actinobacillus pleuropneumoniae and Haemophilus parasuis in Pig by Duplex Droplet Digital PCR

Authors
Wen Wen, Lei Shi, Xun Chen*
Research Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, China
*Corresponding author. Email: cx-wolf@163.com
Corresponding Author
Xun Chen
Received 5 August 2020, Accepted 12 September 2020, Available Online 25 September 2020.
DOI
10.2991/efood.k.200918.001How to use a DOI?
Keywords
Droplet digital PCR; detection; pig; Actinobacillus pleuropneumoniae; Haemophilus parasuis
Abstract

Actinobacillus pleuropneumoniae (A. pleuropneumoniae) and Haemophilus parasuis (H. parasuis) are important pathogens of swine, which cause great economic losses in the swine industry and bring great safety hazards to food safety. In this study, we established a duplex Droplet Digital Polymerase Chain Reaction (ddPCR) assay for sensitive detection of A. pleuropneumoniae and H. parasuis simultaneously in pork. The limit of detection of plasmid DNA could reach 2 copies/μL for both A. pleuropneumoniae and H. parasuis; which were approximately 50- and 20-fold greater sensitivity than Quantitative real-time PCR (qPCR). Both qPCR and ddPCR showed high linearity and positive correlation with standards. The detection results of clinical suspected samples for A. pleuropneumoniae and H. parasuis indicated the positive detection rate of ddPCR (10.7% and 12.5%) was higher than that of qPCR (7.1% and 8.9%). Therefore, the duplex ddPCR assay could be used as an effective quantitative detection to diagnose A. pleuropneumoniae and H. parasuis simultaneously, which may lay a foundation for the development of substitute rapid diagnostic technique.

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Copyright
© 2020 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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<Previous Article In Issue
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Journal
eFood
Volume-Issue
1 - 5
Pages
369 - 376
Publication Date
2020/09/25
ISSN (Online)
2666-3066
DOI
10.2991/efood.k.200918.001How to use a DOI?
Copyright
© 2020 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - JOUR
AU  - Wen Wen
AU  - Lei Shi
AU  - Xun Chen
PY  - 2020
DA  - 2020/09/25
TI  - Simultaneous Detection of Actinobacillus pleuropneumoniae and Haemophilus parasuis in Pig by Duplex Droplet Digital PCR
JO  - eFood
SP  - 369
EP  - 376
VL  - 1
IS  - 5
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.200918.001
DO  - 10.2991/efood.k.200918.001
ID  - Wen2020
ER  -