P81 Antibody to Cardiotonic Steroid Reduces Blood Pressure and Vascular Fibrosis in Preeclampsia

NI Agalakova; VA Reznik; OV Nadei; IA Ershov; OS Rassokha; ML Vasyutina; MM Galagudza; AY Bagrov

doi:10.2991/artres.k.191224.111

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Volume 25, Issue Supplement 1, December 2019, Pages S124 - S124

P81 Antibody to Cardiotonic Steroid Reduces Blood Pressure and Vascular Fibrosis in Preeclampsia

Authors

NI Agalakova³, VA Reznik¹, OV Nadei³, IA Ershov¹, OS Rassokha¹, ML Vasyutina², MM Galagudza², AY Bagrov¹^{, 3}^{, *}

¹Department of Obstetrics and Gynecology, School of Pediatric Medicine

²Institute of Experimental Medicine, Almazov National Medical Research Centre

³Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia

^*Corresponding author. Email: aybagrov@gmail.com

Corresponding Author

AY Bagrov

Available Online 17 February 2020.

DOI: 10.2991/artres.k.191224.111 How to use a DOI?
Abstract: Background: Previous studies implicated cardiotonic steroids, including Na/K-ATPase inhibitor marinobufagenin (MBG), in the pathogenesis of preeclampsia (PE). We demonstrated that MBG induces fibrosis via mechanism involving inhibition of Fli1, a nuclear transcription factor and a negative regulator of collagen-1 synthesis.

Objectives and Methods: We hypothesized that PE blockade of increased MBG with antibody would lessen the fibrosis of umbilical arteries and lower the blood pressure in rats with PE. We tested 36 pregnant Sprague-Dawley rats in which 12 were made hypertensive by 1.8% Na supplementation (days 6–19 of gestation), 12 pregnant rats served controls. At day 19, PE rats received one intraperitoneal injection of polyclonal anti-MBG-4 antibody (0.5 ug/mL) for 4 hours.

Results: PE was associated with higher blood pressure (117 ± 2 vs 107 ± 2 mmHg; p < 0.01), plasma MBG levels (1.54 ± 0.34 vs 0.49 ± 0.11 nmol/L; p < 0.01), protein excretion (26 vs 12 mg/24 hours), sFlt-1 (4-fold), decrease in Fli1 (7-fold) and increase in collagen-1 in aorta (4-fold) vs. control rats (all p < 0.01). In 12 rats was treated with polyclonal anti-MBG-4 antibody blood pressure dropped (93 ± 3 mmHg) and Fli1 was decreased much less (2-fold; p < 0.01 vs nontreated rats).

Conclusion: These results demonstrate that in experimental PE elevated MBG level is implicated in umbilical fibrosis via suppression of Fli1. Supported by Russian Scientific Foundation grant № 18-15-00222.
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal: Artery Research
Volume-Issue: 25 - Supplement 1
Pages: S124 - S124
Publication Date: 2020/02/17
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.2991/artres.k.191224.111 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - NI Agalakova
AU  - VA Reznik
AU  - OV Nadei
AU  - IA Ershov
AU  - OS Rassokha
AU  - ML Vasyutina
AU  - MM Galagudza
AU  - AY Bagrov
PY  - 2020
DA  - 2020/02/17
TI  - P81 Antibody to Cardiotonic Steroid Reduces Blood Pressure and Vascular Fibrosis in Preeclampsia
JO  - Artery Research
SP  - S124
EP  - S124
VL  - 25
IS  - Supplement 1
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.191224.111
DO  - 10.2991/artres.k.191224.111
ID  - Agalakova2020
ER  -

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