Proceedings of the 5th Universitas Ahmad Dahlan Public Health Conference (UPHEC 2019)

Molecular Resistance Status of Aedes aegypti to the Organophosphate and Pyrethroid Insecticides in Central Sulawesi and East Nusa Tenggara Provinces, Indonesia

Authors
Muhammad Choirul Hidajat, Edi Dharmana, Mega Tyas Prihatin, Martini, Triwibowo Ambargarjito
Corresponding Author
Muhammad Choirul Hidajat
Available Online 20 March 2020.
DOI
10.2991/ahsr.k.200311.023How to use a DOI?
Keywords
Aedes aegypti, resistance, VGSC, AChE, Central Sulawesi, East Nusa Tenggara
Abstract

Currently, dengue has became a major public health problem in Indonesia. Aedes aegypti is confirmed as the main dengue vector. The organophosphate and phyretroid have been used in vector control program for more than 3 decades. Insecticide resistance evidences and mechanisms are essential to find the current status of insecticide susceptibility of dengue vectors. In this study, we analyzed the molecular principles of resistance to phyrethroid and organophosphate insecticides on mosquitoes collected from Palu, Central Sulawesi, and Belu and Ende, East Nusa Tenggara. Single-step polymerase chain reaction (PCR) method was conducted to detect amino acid mutations in paratype voltage-gated sodium channel (VGSC) gene and Achetylcoline esterase-1 (AChE) gene of Ae. Aegypti mosquitoes. The result showed that V1016G mutations of VGSC gene were detected from the wild-caught Ae. Aegypti mosquito from Palu, Belu and Ende. In contrast, G119 wild type allele of AChE gene was found from all Ae. Aegypti of all study sites. These evidences suggest that Ae. aegypti from Palu, Belu and Ende have developed multiple resistance towards phyrethroid insecticides. Based on prior susceptibility test, Ae. aegypti from all study sites were possibly developing resistance to organophosphate in other mechanisms. New strategies are needed, especially insecticide rotation in this area to achieve efficient vector control.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 5th Universitas Ahmad Dahlan Public Health Conference (UPHEC 2019)
Series
Advances in Health Sciences Research
Publication Date
20 March 2020
ISBN
978-94-6252-932-8
ISSN
2468-5739
DOI
10.2991/ahsr.k.200311.023How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Muhammad Choirul Hidajat
AU  - Edi Dharmana
AU  - Mega Tyas Prihatin
AU  - Martini
AU  - Triwibowo Ambargarjito
PY  - 2020
DA  - 2020/03/20
TI  - Molecular Resistance Status of Aedes aegypti to the Organophosphate and Pyrethroid Insecticides in Central Sulawesi and East Nusa Tenggara Provinces, Indonesia
BT  - Proceedings of the 5th Universitas Ahmad Dahlan Public Health Conference (UPHEC 2019)
PB  - Atlantis Press
SP  - 122
EP  - 127
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200311.023
DO  - 10.2991/ahsr.k.200311.023
ID  - Hidajat2020
ER  -