Construction and Identification of Eukaryotic Expression Vector of Vlgr1

DOI

10.2991/sser-17.2018.46How to use a DOI?

Keywords

Vlgr1; Vector; Construction; Identification; Expression

Abstract

To construct the Vlgr1 plasmid containing the functional domain, provide experimental material for the study of Vlgr1 - mediated signaling pathways and hydrolysis mechanisms. Methods: Primers were designed according to the gene sequences in the Gene bank, the DNA sequences of different domain regions were cloned and inserted into vector -pEGFP -N1 to construct Vlgr1--pEGFP -N1 expression plasmid, and then transfected into 293T cell line by double digestion, PCR and sequencing. The transfection was detected by fluorescence and Western Blot, the expression of Vlgr1 gene was observed. Results: It was confirmed that the plasmid was successfully expressed by gene sequencing, fluorescence method and Western Blot method. Conclusions: The Vlgr1 plasmid containing the functional domain was successfully constructed.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Zeng Ping
AU  - Huang Xiaoming
AU  - Cheng Xueli
AU  - Wang Anping
AU  - Zhao Chunling
AU  - Dong Junhong
PY  - 2018/01
DA  - 2018/01
TI  - Construction and Identification of Eukaryotic Expression Vector of Vlgr1
BT  - Proceedings of the 2017 7th International Conference on Social science and Education Research (SSER2017)
PB  - Atlantis Press
SP  - 219
EP  - 224
SN  - 2352-5398
UR  - https://doi.org/10.2991/sser-17.2018.46
DO  - 10.2991/sser-17.2018.46
ID  - Ping2018/01
ER  -