Stability and antioxidant tests of ethanol extract liposome of moringa leaves (Moringa oleifera)

DOI

10.2991/978-94-6463-322-1_22How to use a DOI?

Keywords

antioxidant; ethanol extract; liposome; moringa leaf

ABSTRACT

Moringa leaf potentially has an antioxidant effect because it contains Quercetin having poorsolubility in water. Liposomes as carriers of drug compounds can increase the solubility of quercetin through an entrapment system in the lipid bilayer. This study aimed to determine the stability and antioxidant activity of ethanol extract liposome of moringa leaves. This research method used experimental research utilizing moringa leaf ethanol extract in different concentrations (0,125%, 0.25%, and 0.5%) which was formulated into phospholipids by extrusion method. Preparation of liposome utilized Lipoid S-75 and cholesterol (1:1) by ethanol injection method then was characterized and stability tested. Meanwhile, the antioxidant test of ethanol extract liposome of moringa leaves used DPPH assay. The characterization results showed that particle size of moringa leaf ethanol extract (0.125%, 0.25%, 0.5%) liposomes were 112, 145, and 175 nm, with polydispersity index (PDI) of 0.34, 0.28, 0.12 respectively. Moreover, the entrapment efficiency (%EE) of all formulations showed that 0.25% of moringa leaf ethanol extract liposome had the highest load into liposome (93%) compared to 0.125% (80%) and 0.5% (60%)only the best result (0.25%) of size, %EE and stability which continued for the antioxidant test. Six weeks of stability study described that size and retention profile of liposomes were stable at 25 ℃ and 37 ℃ (0.25%). The result of the antioxidant test was obtained by inhibitory concentration (IC₅₀) 124.41 ppm (only ethanol extract) and 61.78 ppm (loaded-liposome) which was included in the moderate and strong category respectively. It can be concluded that moringa leaf ethanol extract liposome has strong antioxidant activity as a promising nano nutraceutical formulation.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Robert Tungadi
AU  - Teti Sutriyati Tuloli
AU  - Sri Manovita Pateda
PY  - 2023
DA  - 2023/12/30
TI  - Stability and antioxidant tests of ethanol extract liposome of moringa leaves (Moringa oleifera)
BT  - Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
PB  - Atlantis Press
SP  - 165
EP  - 169
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-322-1_22
DO  - 10.2991/978-94-6463-322-1_22
ID  - Tungadi2023
ER  -