Proceedings of the 1st Nusa Tenggara International Conference on Chemistry (NITRIC 2022)

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Characterization of Organic Solvent Tolerance Lipase from Compost Indigenous Bacteria

Authors
Nurhasanah1, *, Aspita Laila1, Heri Satria1, Ni Luh G. R. Juliasih1, Qonita Nurul Husna1
1Department Chemistry, Faculty Mathematics and Natural Science, University of Lampung, Bandar Lampung, Indonesia
*Corresponding author. Email: nur.hasanah@fmipa.unila.ac.id
Corresponding Author
Nurhasanah
Available Online 14 April 2023.
DOI
10.2991/978-94-6463-130-2_4How to use a DOI?
Keywords
lipase; organic solvent; compost; indigenous bacteria
Abstract

Lipases are a group of hydrolase enzymes that play an important role in industry and biotechnology. In addition, lipase has the ability as a catalyst in ester hydrolysis, transesterifications, alcoholysis, and acydolysis reactions. This study aims to obtains the character of the tolerant lipase of organic solvents from the compost indigenous bacteria. The method used in determining the optimum conditions for lipase production, purification, and characterization of tolerant lipase in organic solvent. Lipase activity was tested by a spectrophotometric method using p-nitrophenyl palmitate as a substrate and protein content was determined using the Lowry method. The result showed that the compost indigenous bacteria could produce lipase tolerant of organic solvents at the optimum growth conditions of 48 h incubations time, pH 6, inducer concentrations of 2% with the addition of 3% hexane. The specific activity of lipase produces a value of 1455.68 U/mg. Purifications by fractionation of ammonium sulfate and dialysis increased the purity of the lipase 2.37 times with a specific activity of 3449.65 U/mg. Lipase activity increased with the addition of metal ions Ba+2 and Al+3 and n-hexane as solvent. Meanwhile, the presence of metal ions Na+, K+, Mg2+ Fe+3 as well as methanol and benzene solvents affected decreasing lipase activity.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 1st Nusa Tenggara International Conference on Chemistry (NITRIC 2022)
Series
Atlantis Highlights in Chemistry and Pharmaceutical Sciences
Publication Date
14 April 2023
ISBN
978-94-6463-130-2
ISSN
2590-3195
DOI
10.2991/978-94-6463-130-2_4How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Nurhasanah
AU  - Aspita Laila
AU  - Heri Satria
AU  - Ni Luh G. R. Juliasih
AU  - Qonita Nurul Husna
PY  - 2023
DA  - 2023/04/14
TI  - Characterization of Organic Solvent Tolerance Lipase from Compost Indigenous Bacteria
BT  - Proceedings of the 1st Nusa Tenggara International Conference on Chemistry (NITRIC 2022)
PB  - Atlantis Press
SP  - 20
EP  - 29
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-130-2_4
DO  - 10.2991/978-94-6463-130-2_4
ID  - 2023
ER  -