Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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Molecular Identification of Bone Morphogenetic Protein-15 (BMP-15) gene of Sumba Ongole cattle

Authors
Cynthia Dewi Gaina1, *, Maxs U.E. Sanam1, WMN Nalley2, Imanuel Benu2
*1Faculty of Veterinary Medicine, University of Nusa Cendana, Kupang, Indonesia
2Faculty of Animal Science, University of Nusa Cendana, Kupang, Indonesia
*Corresponding author. Email: cynthia.gaina@staf.undana.ac.id
Corresponding Author
Cynthia Dewi Gaina
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.070How to use a DOI?
Keywords
BMP-15; genes; Sumba; Ongole; Cattle
Abstract

This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
978-94-6239-557-2
ISSN
2468-5747
DOI
10.2991/absr.k.220309.070How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Cynthia Dewi Gaina
AU  - Maxs U.E. Sanam
AU  - WMN Nalley
AU  - Imanuel Benu
PY  - 2022
DA  - 2022/03/29
TI  - Molecular Identification of Bone Morphogenetic Protein-15 (BMP-15) gene of Sumba Ongole cattle
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 361
EP  - 364
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.070
DO  - 10.2991/absr.k.220309.070
ID  - Gaina2022
ER  -