Genetic markers of antibiotic resistance of pathogenic bacteria in the milk of cows and goats

DOI

10.2991/ispc-19.2019.11How to use a DOI?

Keywords

resistance, gene, DNA, antimicrobial drugs, bacterial pathogens, genetic determinants, milk

Abstract

This article represents the results of studies of the microorganism resistance to antimicrobial agents detected by PCR in the milk of cows and goats. In 94.1% of cow milk and in 80.7% of goat milk samples specific DNA regions of microorganisms were detected: Staphylococcus spp., E. coli, S. aureus, S. agalactiae. The more common pathogenic agents were the bacteria of the Staphylococcus spp. group (S. epidermidis, S. saprophyticum, S. haemolyticus) and E. coli. The opportunistic bacteria of the Staphylococcus spp. group most often were carriers of the ErmB gene, which is responsible for resistance to macrolides of the 1st generation. E. coli had a high percentage of resistance to inhibitor-protected penicillin and cephalosporin of the 3rd and 4th generations. Acquired resistance of E. coli and bacteria of the Staphylococcus spp. group (S. epidermidis, S. saprophyticum, S. haemolyticus) is associated with the frequent use of antimicrobials in the agricultural enterprises, such as penicillin, 3rd and 4th generation cephalosporin and 1st generation macrolides. Also the poly-resistant E. coli isolates (2.2%) resistant to several groups of antimicrobial drugs were detected in the cow milk samples.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Natalia Bezborodova
AU  - Olga Sokolova
AU  - Marina Ryaposova
AU  - Maria Isakova
PY  - 2019/06
DA  - 2019/06
TI  - Genetic markers of antibiotic resistance of pathogenic bacteria in the milk of cows and goats
BT  - Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019)
PB  - Atlantis Press
SP  - 44
EP  - 48
SN  - 1951-6851
UR  - https://doi.org/10.2991/ispc-19.2019.11
DO  - 10.2991/ispc-19.2019.11
ID  - Bezborodova2019/06
ER  -