A Potential Yeast Strains for Biological Control of Mosquitoes

DOI

10.2991/absr.k.210810.023How to use a DOI?

Keywords

antagonistic yeast, insects, Candida sp, Jaminaea sp

Abstract

Malaria is one of the diseases that have not been successfully eradicated, and are still a threat in many countries. Efforts have been made to eradicate vector-borne diseases, including the use of insecticides and physical approaches, which are proven not effective in overcoming the malaria problem. One of the recent approach to overcome the problem is employing endosymbiotic microorganisms for killing the mosquitoes as malaria vector. Antagonistic yeast that inhibit the growth of other organisms has been reported from various habitats, including the digestive tract of insects. It is then the purpose of this study to isolate potential yeast(s) as an antagonistic yeast from the digestive tract of mosquitoes. Local mosquitoes were used as samples, dissected and a serial platting was done for isolating the yeast. The obtained isolate(s) were tested for its antagonistic activity against a sensitive strain, Saccharomyces cerevisiae CEN-PK2-1C, and resulting in two isolates which showed consistent antagonistic activities. Molecular identification were done by employing the Internal Transcription Spacer (ITS) area as a marker gene. An isolate, namely NY24 showed the highest similarity with Jaminaea sp, while the NY38 isolate is located in the same cluster with Candida sp group. However, it is noteworthy to mention that both isolates are separated from what is known as VLE-yeast killer group, and deserve further research as potential candidates for mosquitoes’ biocontrol agent, especially in Indonesia.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - D Satwika
AU  - V R A Permatasari
AU  - G E N Cahyani
PY  - 2021
DA  - 2021/08/12
TI  - A Potential Yeast Strains for Biological Control of Mosquitoes
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 119
EP  - 125
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.023
DO  - 10.2991/absr.k.210810.023
ID  - Satwika2021
ER  -