Characterization of Bacteriocin Produced by Bacillus subtilis 11A Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus)
- DOI
- 10.2991/absr.k.210810.021How to use a DOI?
- Keywords
- Antimicrobial activity, Bacillus subtilis, Bacteriocin, Gallus domesticus, Stability
- Abstract
The purpose of this study was to characterize bacteriocins produced by Bacillus subtilis 11A isolated from Indonesian native chicken’s ileum. Characterization of bacteriocins included antimicrobial activity, the stability to temperature, pH, and storage time. Antimicrobial activity was tested against Escherichia coli FNCC 0091, Salmonella typhimurium FNCC 0134, and Staphylococcus aureus FNCC 0143. Stability to temperature was tested to 4, 30, 70, 80, 90, 100oC for an hour and 121oC for 15 min. Stability to pH was tested to pH 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 for an hour. Stability to storage was tested for 7, 14, 21, and 28 days at refrigerator (4oC) and room temperature (30oC). The result showed that bacteriocins produced by Bacillus subtilis 11A were more effective against pathogens Gram-negative (Escherichia coli and Salmonella typhimurium) than Gram-positive (Staphylococcus aureus) (P<0.05). The bacteriocins produced by Bacillus subtilis 11A were heat until 90oC, stable at pH 6–10 and stored at refrigerator up to 28 days (P<0.05). This study suggested that this bacteriocins might become potential candidate for use as biodegradable natural fed and alternative antimicrobial agents to solve the increasing trends of problems of antibiotic resistance.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - V A Cahya AU - C Hanim AU - L M Yusiati PY - 2021 DA - 2021/08/12 TI - Characterization of Bacteriocin Produced by Bacillus subtilis 11A Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus) BT - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019) PB - Atlantis Press SP - 110 EP - 114 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210810.021 DO - 10.2991/absr.k.210810.021 ID - Cahya2021 ER -