Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island

DOI

10.2991/absr.k.210810.040How to use a DOI?

Keywords

Xylanase purification, Aspergillus tamari TKM-24

Abstract

Utilizing of chlorine-based chemicals in pulp bleaching process causes environmental problem. Utilization of biological agents, such as enzymes for bleaching process is a wise measure to reduce the problem. One of the enzymes that play an important role in pulp bleaching process is xylanase. The previous conducted research found out xylanolyitic fungi and determined for while as Aspergillus tamari TKM-24. The purpose of research was to purify xylanase enzyme t. The research methods were partial optimization of xylanase production and purification its xylanase Aspergillus tamari TKM-24. Partial optimization including environmental factors (pH, agitation, temperature, airation), inoculum, substrate concentration, and time incubation. Purification steps of xylanase were precipitation by ammonium sulfate and gel filtration on sephadex G-75. Characterization of purified xylanase consisted of molecular weight, effect of pH and temperature to xylanase activity. The results showed that optimization of xylanase production was obtained by inoculum concentration 1 % (v/v), substrate xylan concentration 1% (w/v), pH 5 and incubation time for 4 days. Xylanase showed a single band on SDS-PAGE analysis with a moleculer mass 25 kDa and was purified 10,2 - fold by Spadex G-75. The Km and Vmax values were 24,4 mg/ml and 1520 μmol/ml/min for oat spelt xylan, respectively. The Xylanase was clasified as termotolerant which had optimal temperature was around 55o C and pH value 6.0.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - S Margino
AU  - Y Mallisa
AU  - W Asmara
PY  - 2021
DA  - 2021/08/12
TI  - Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 234
EP  - 244
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.040
DO  - 10.2991/absr.k.210810.040
ID  - Margino2021
ER  -