Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli

DOI

10.2991/absr.k.210810.028How to use a DOI?

Keywords

amylolytic index, inasua, MRSA, pH optimum

Abstract

Bacteriocin-producing Lactobacilli have been successfully isolated from inasua, a fermented fish product from Teon, Tilapia, and Serua, Province of Maluku Indonesia. In addition to producing bacteriocin, Lactobacilli could produce amylase enzymes. This study aims to characterize crude amylase enzyme activity from bacteriocin-producing Lactobacillus plantarum ITN13 and Lactobacillus rhamnosus IN13 based on pH and temperature optimum enzyme activity. Both isolates were grown on media de Mann Rogosa Sharpe Agar (MRSA) + 1% starch and media Nurient Agar (NA) + 1% starch. Amylolytic index (AI) of L. plantarum ITN13 and L. rhamnosus IN13 were 1.33 and 1.00, respectively. Amylase production of L. rhamnosus IN13 maximum at 3 hours incubation was 0.123 (Unit / mg protein) while L. plantarum ITN13 at 6 hours was 0.092 (Unit / mg protein) in the exponential phase, respectively The optimum amylase activity from L. rhamnosus IN13 at 45oC and pH 5, while the optimum of amylase L. plantarum ITN13 at 35oC and pH 6.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - NR Mubarik
AU  - HA Surya
AU  - Suryani
PY  - 2021
DA  - 2021/08/12
TI  - Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 153
EP  - 157
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.028
DO  - 10.2991/absr.k.210810.028
ID  - Mubarik2021
ER  -