Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz.
- DOI
- 10.2991/isils-19.2019.88How to use a DOI?
- Keywords
- P. fruticosa L. herb, RP HPLC, quercetin glycosides, kaempferol glycosides, rhamnetin glycosides, proacyanidins, elagic acid, ellagotannins
- Abstract
The purpose of this study was to develop a method of quali-tative and quantitative assessment of biologically active sub-stances of P. fruticosa L. herb. As object of study was used P. fruticosa L. herb. We used the method of high performance liquid chromatography in reversed-phase variant. As a result of chromatography, it was found that the chromatographic system used can effectively separate and identify the poly-phenol complex of the herb P. fruticosus L. The chromato-gram shows that the polyphenol complex of the herb P. fruti-cosus L. consists of the glycosides quercetin, kaempferol, rhamnetin, procyanidins, ellagotannins and ellagic acid and chlorogenic acid. Aglycones were found in the hydrolysis products: quercetin, kempferol, ellagic acid, and trace amounts of rhamnetin. Thus, as a result of this study, the composition of the polyphenolic complex of the herb P. fruti-cosus L. was established, including the glycosides of querce-tin, kaempferol, rhamnetin, proacyanidins, ellagic acid and ellagotannins. A method has been developed for the quanti-tative determination of the sum of aglycones of grass poly-phenols of P. fruticosus L., which is based on the principle of extraction with the simultaneous hydrolysis of glycosides of polyphenols to aglycones and their subsequent chromato-graphic determination by high performance liquid chroma-tography and quantification using absolute calibration.
- Copyright
- © 2019, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Elena Zhilyakova AU - Oleg Novikov AU - Dmitriy Pisarev AU - Liliya Zolotareva PY - 2019/11 DA - 2019/11 TI - Development of Methods For Quantitative Determination of Polyphenols in Grass Pentaphylloides Fruticosa (L.) O. Schwarz. BT - Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019) PB - Atlantis Press SP - 89 EP - 93 SN - 2468-5747 UR - https://doi.org/10.2991/isils-19.2019.88 DO - 10.2991/isils-19.2019.88 ID - Zhilyakova2019/11 ER -