Optimization of a Method for Obtaining and Determination of Carotenoids in Tambukan Mud

DOI

10.2991/isils-19.2019.37How to use a DOI?

Keywords

Tambukan lake, silt mud, ultrasound-assisted extraction, carotenoids, spectrophotometry

Abstract

Therapeutic sulphide silt mud from Tambukan lake has been formed for a long period of time. Balneological value of silt mud from that specific lake is high because of high level of ferrous sulphides and water-soluble salts in it’s content, including recognized as therapeutically active bromine and boric acid; high concentration of lipids and carotenoids, which have anti-inflammatory, anti-bacteria, immune stimulating and bio stimulating effects. Goal: development of the method for stimulation of the peloid extract obtaining process from the Tambukan mud by an ultrasound-assisted extraction with further determination of biologically active substance using a spectrophotometric method. The research is based on formerly known and patented methods, offered by Kh.G.Karagulov. The method is reasonable, allows to extract carotenoid in full. The disadvantage of it is the energy-demanding and the usage of an organic solvent. As the object of the research a deep seated mud sample from the Tambukan lake was taken. After an ultrasound-assisted extraction there was obtained an oily extract from the mud sample. Quantitative content estimation was held out using a spectrophotometric method in visible region of absorption. The ability to optimize the process of peloid extraction from the Tambukan mud by the ultrasound-assisted extraction has been proven. Offered method for extraction and determination of carotenoid amount in Tambukan mud is less resource- and energy-demanding. Alongside with that it provides best possible carotenoid amount in the outcome. Effective and less expensive method to obtain peloid from Tambukan mud has been developed. The research results point out that the level of carotenoids in Tambukan silt mud has been reduced resulting in serious reduction of therapeutic effect.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dmitriy Kompantsev
AU  - Anna Chakhirova
AU  - Taisiya Gutnova
PY  - 2019/11
DA  - 2019/11
TI  - Optimization of a Method for Obtaining and Determination of Carotenoids in Tambukan Mud
BT  - Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)
PB  - Atlantis Press
SP  - 258
EP  - 261
SN  - 2468-5747
UR  - https://doi.org/10.2991/isils-19.2019.37
DO  - 10.2991/isils-19.2019.37
ID  - Kompantsev2019/11
ER  -