Crude Aflatoxin B₁ Production Using Maize and Rice Substrates for Animal Research

DOI

10.2991/absr.k.220401.004How to use a DOI?

Keywords

Aflatoxin B1; Production; Substrat; Maize dan Rice

Abstract

This study aimed to develop a simple method of crude aflatoxin (AF) B₁ production for animal research. A. flavus was planted on potato dextrose agar (PDA) medium as an isolate to be grown on maize and rice substrates at a concentration of 107/mL. Maize was added to water until the water content reached 25%, while rice was soaked in water at a ratio of 1 mL of water for 2 g of rice for 1 hour. The rice and maize were sterilized using an autoclave. A total of 250 g of each substrate was inoculated with A. flavus FNCC 6122. Incubation was carried out for 6 days at 30°C at a humidity of 80-90%. On the second day of the incubation period, 1 mL of water was added to the substrates. At the end of the incubation period, crude AF samples were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that level of AFB₁ on rice higher than maize (P<0.001). The AFB₁ production was 3229 µg/kg in rice and 1153 µg/kg in maize. Rice and maize can be used as a substrate source for crude AFB₁ production for animal research.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Muhsin Al Anas
AU  - Lies Mira Yusiati
AU  - Cuk Tri Noviandi
AU  - Ali Agus
PY  - 2022
DA  - 2022/04/11
TI  - Crude Aflatoxin B₁ Production Using Maize and Rice Substrates for Animal Research
BT  - Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
PB  - Atlantis Press
SP  - 17
EP  - 21
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220401.004
DO  - 10.2991/absr.k.220401.004
ID  - AlAnas2022
ER  -