Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)

The Ks and µmax Determination of Proteolytic Lactic Acid Bacteria Isolated from Colostrum of Dairy Cattle with Differences in Energy Source Levels

Authors
Yuhud Agung Nugroho1, Zaenal Bachruddin1, *, Widodo Widodo1, Yuny Erwanto1, Chusnul Hanim1
1Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
*Corresponding author. E-mail: bachrudin@ugm.ac.id
Corresponding Author
Zaenal Bachruddin
Available Online 11 April 2022.
DOI
10.2991/absr.k.220401.005How to use a DOI?
Keywords
Lactic acid bacteria; Ks (substrate affinity constant); Define medium
Abstract

This study determines the optimal substrate-level used by proteolytic acid lactic bacteria to produce a product expressed in substrate affinity constant (Ks). Proteolytic lactic acid bacteria were isolated from cow colostrum, carried out at the Laboratory of Nutritional Biochemistry, Faculty of Animal Science, Universitas Gadjah Mada. Proteolytic lactic acid bacteria was rejuvenated first in a solid medium, and the amount is multiplied in MRS broth medium to be used as stock. Proteolytic lactic acid bacteria was observed for growth on a defined medium with five variations of glucose substrate levels. The glucose levels were given at 0.15%, 0.1%, 0.05%, 0.01%, and 0% with three replications and one negative control at each level. Proteolytic lactic acid bacteria were incubated at 37°C for 24 hours. Observations were made every two hours. The results of the absorbance observations were used to calculate the Ks value. The Ks value was used to determine the substrate level in the next liquid fermentation. The Ks and µmax values obtained in the proteolytic lactic acid bacteria were 0.0569 gram/100 ml and 0.0125 per hour, respectively.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
Series
Advances in Biological Sciences Research
Publication Date
11 April 2022
ISBN
978-94-6239-562-6
ISSN
2468-5747
DOI
10.2991/absr.k.220401.005How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

TY  - CONF
AU  - Yuhud Agung Nugroho
AU  - Zaenal Bachruddin
AU  - Widodo Widodo
AU  - Yuny Erwanto
AU  - Chusnul Hanim
PY  - 2022
DA  - 2022/04/11
TI  - The Ks and µmax Determination of Proteolytic Lactic Acid Bacteria Isolated from Colostrum of Dairy Cattle with Differences in Energy Source Levels
BT  - Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
PB  - Atlantis Press
SP  - 22
EP  - 25
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220401.005
DO  - 10.2991/absr.k.220401.005
ID  - Nugroho2022
ER  -