Functional evaluation of bacterial surface displayed P domain from human norovirus capsid proteins

DOI

10.2991/imst-16.2016.41How to use a DOI?

Keywords

human norovirus; P domain; cell surface display; receptor binding properties.

Abstract

Human noroviruses (HuNoVs) are the major cause to acute nonbacterial gastroenteritis outbreaks. The receptors of HuNoVs are difficult to identify because HuNoVs cannot be easily cultivated in vitro and they lack effective animal models. To develop an effective method for mining functional viral receptors, engineering Escherichia coli were constructed to display P domain of capsid protein of HuNoVs (GI.1 or GII.4) on the cell surface, using the N-terminal of the ice nucleation protein (InaQN). In this study, membrane protein-porcine gastric mucin binding ELISA methods were employed to confirm the surface display efficiency. The receptor binding capacity of InaQN-P (GI.1 or GII.4) fusion proteins on the bacterial surface was evaluated through whole-cell fluorescence immunoassay with saliva-based histo-blood group antigens. Results revealed remarkable characteristic of antigenicity and receptor binding properties. Therefore, this bacterial surface display system should be considered as a candidate to mine and investigate HuNoV receptors in vivo or in vitro.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Shaofeng Rong
AU  - Yue Zhou
AU  - Mengya Niu
AU  - Qianqian Li
AU  - Ming Wang
AU  - Shimin Guan
AU  - Shuo Zhang
AU  - Baoguo Cai
PY  - 2016/11
DA  - 2016/11
TI  - Functional evaluation of bacterial surface displayed P domain from human norovirus capsid proteins
BT  - Proceedings of the 2016 International Conference on Innovative Material Science and Technology (IMST 2016)
PB  - Atlantis Press
SP  - 277
EP  - 284
SN  - 1951-6851
UR  - https://doi.org/10.2991/imst-16.2016.41
DO  - 10.2991/imst-16.2016.41
ID  - Rong2016/11
ER  -