Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

DOI

10.2991/ifeesm-15.2015.133How to use a DOI?

Keywords

Cunninghamia lanceolata,Chinese fir ,water deficit, proteome.

Abstract

Chinese fir (Cunninghamia lanceolata) is an important commercial timber species in China. In the recent years, seasonal droughts occurred frequently in the fir growing areas and made a great impact on its production. In this study, PEG-6000 was used to simulate water deficit, while the drought-resistant FS43 and drought sensitive FS38 fir strains were selected as experimental materials. A two-dimensional electrophoresis technology was used for proteome analysis of leave protein comparisons between the two fir strains under water deficit. A total number of 24 differentially expressed protein spots were detected, of which five were identified by the tandem mass spectrometry and divided into four categories: (a) Functional enzymes involved in photosynthesis: the light-harvesting chlorophyll a/b-binding proteins, ribulose-1, 5-bisphosphate carboxylase/oxygenase; (b) Functional enzymes involved in osmotolerance: myo-inositol 1-phosphate synthase; (c) Functional enzymes involved in carbohydrate metabolism: granule-bound starch synthase I; (d) A conifer specific antioxidant stress-related functional enzyme. Under water deficit, these enzymes with obvious differences in protein expression levels might be involved in the regulation of fir responses to seasonal drought stress.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guochang Ding
AU  - Weizhi Guo
AU  - Shaoning Ruan
AU  - Shubin Li
AU  - Yu Chen
AU  - Sizu Lin
PY  - 2015/09
DA  - 2015/09
TI  - Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit
BT  - Proceedings of the 2015 International Forum on Energy, Environment Science and Materials
PB  - Atlantis Press
SP  - 714
EP  - 723
SN  - 2352-5401
UR  - https://doi.org/10.2991/ifeesm-15.2015.133
DO  - 10.2991/ifeesm-15.2015.133
ID  - Ding2015/09
ER  -